We narrowed to 58,072 results for: SAP

1. pLentiCRISPRv1-CENPB

   Plasmid

   #120208

   Purpose

   gRNA targeting the stem loop of CENPB poly(A) site

   Depositor

   David Bartel

   Article

   Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

   Insert

   CENPB poly(A) site
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDK362

   Plasmid

   #134194

   Purpose

   Retroviral plasmid with SKAP insert

   Depositor

   Iain Cheeseman

   Insert

   SKAP 166-316-LAP with G5 and Jens siRNA hardening (KNSTRN Human)
   

   Use

   Retroviral

   Tags

   LAP (GFP)

   Expression

   Mammalian

   Mutation

   aa 166-316 with G5 and Jens siRNA hardening

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKLF6.1.0-gDNA

   Plasmid

   #132435

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   KLF6 (KLF6 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGD252

   Plasmid

   #132373

   Purpose

   contains the sequence of TSapphire for C-term fusions in the pGGD000 backbone (GreenGate)

   Depositor

   Rüdiger Simon

   Article

   Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

   Insert

   linker-TSapphire
   

   Use

   Greengate entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ad-EEF1D-S133E

   Plasmid

   #131577

   Purpose

   Expresses EEF1D with S133E mutation in mammalian cells

   Depositor

   Haiyan Xu

   Insert

   Eukaryotic translation elongation factor 1 delta (EEF1D Human)
   

   Use

   Adenoviral

   Tags

   No

   Expression

   Mutation

   changed Serine 133 to Glutamic acid

   Promoter

   CMV

   Available since

   Oct. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a human Tankyrase2 full length Y920E

   Plasmid

   #132639

   Purpose

   expresses human Tankyrase2 full length Y920E in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

   Insert

   Tankyrase2 Y920E
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. JB11

   Plasmid

   #128916

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab18QL-BirA*-HA-MAO (RAB18 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. JB10

   Plasmid

   #128917

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab18SN-BirA*-HA-MAO (RAB18 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. JB2

   Plasmid

   #128899

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab2ASN-BirA*-HA-MAO (RAB2A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. JB98

    Plasmid

    #128927

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    RalBSA-BirA*-HA-MAO (RALB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3.1-V5-SPR

    Plasmid

    #127236

    Purpose

    Eukaryotic expression of SPR

    Depositor

    Kai Johnsson

    Article

    Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

    Insert

    SPR (SPR Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pE2F3-donor

    Plasmid

    #113809

    Purpose

    CRISPR donor plasmid to tag human transcription factor E2F3 with GFP

    Depositor

    Kevin White

    Insert

    E2F3 homology arms flanking EGFP-IRES-Neo cassette (E2F3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Unknown

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide2

    Plasmid

    #118159

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA2 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide3

    Plasmid

    #118160

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA3 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOZ-FH-C Bach1 K52R

    Plasmid

    #73645

    Purpose

    Retroviral expression of Bach1 K52Rmutant (helicase null)

    Depositor

    Sharon Cantor

    Insert

    Bach1 (BACH1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and His

    Expression

    Mammalian

    Mutation

    K52R (helicase null)

    Promoter

    unknown

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pNR2F1.1.0-gDNA

    Plasmid

    #112484

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1

    Depositor

    Kevin White

    Insert

    NR2F1 (NR2F1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSMAD3.1.0-gDNA

    Plasmid

    #112407

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SMAD3

    Depositor

    Kevin White

    Insert

    SMAD3 (SMAD3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pELF4-donor

    Plasmid

    #112371

    Purpose

    CRISPR donor plasmid to tag human transcription factor ELF4 with GFP

    Depositor

    Kevin White

    Insert

    ELF4 homology arms flanking EGFP-IRES-Neo cassette (ELF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVEZF1-donor

    Plasmid

    #112341

    Purpose

    CRISPR donor plasmid to tag human transcription factor VEZF1 with GFP

    Depositor

    Kevin White

    Insert

    VEZF1 homology arms flanking EGFP-IRES-Neo cassette (VEZF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs999907002, hg38:17:57975308:A>T, hg38:17:579…

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNR1D2-donor

    Plasmid

    #112314

    Purpose

    CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP

    Depositor

    Kevin White

    Insert

    NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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