We narrowed to 11,712 results for: NSI

1. pDONR223 SARS-CoV-2 ORF9Bwu

   Plasmid

   #149331

   Purpose

   Gateway-compatible Entry vector, with insert of ORF9Bwu gene's CDS from SARS-CoV-2 genome definition in Wu et al, Nature 2020

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF9Bwu
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGLS3-5xHRE-p53(K120R/K164R)

   Plasmid

   #72556

   Purpose

   Hypoxia induced mutant p53. Contains the HRE from VEGF sequence (5 repeats) upstream of p53 K120R/K164R.

   Depositor

   Ester Hammond

   Article

   Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

   Insert

   p53 (TP53 Human)
   

   Tags

   5XHRE

   Expression

   Mammalian

   Mutation

   K120R/K164R

   Promoter

   E1B minimal promoter

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-nEF-Con/Foff 2.0-ChR2-mCherry

   Plasmid

   #137143

   Purpose

   Intersectional viral expression of ChR2-mCherry in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-ChR2-mCherry
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   H134R

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-TNT-CFP-STIM1-C227W

   Plasmid

   #68403

   Purpose

   Expresses a STIM1 gain-of-function mutant in mammalian cells

   Depositor

   Yubin Zhou

   Article

   Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

   Insert

   CFP-STIM1 (STIM1 Human)
   

   Tags

   Cyan Fluorescent Protein (CFP)

   Expression

   Mammalian

   Mutation

   Changed Cysteine 227 to Tryptophan; CFP was inser…

   Promoter

   CMV

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. MSCV-BRD9_BRD7BD-PGK-Puro-IRES-GFP

   Plasmid

   #75118

   Purpose

   Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the BRD7 bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Mutation

   Bromodomain replaced with the bromodomain of BRD7

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pDONR223 SARS-CoV2 TEV-NSP12

   Plasmid

   #154410

   Purpose

   Gateway-compatible Entry vector

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector, with insert of n…

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   July 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pmiRFP-beta-Actin-C-19

   Plasmid

   #108412

   Purpose

   Beta-actin fused to monomeric near-infrared fluorescent protein miRFP

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Insert

   beta-Actin
   

   Tags

   miRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4.27_eSOX17

   Plasmid

   #195498

   Purpose

   Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   Full distal endoderm enhancer region of SOX17 (SOX17 Human)
   

   Tags

   minP_luc2-hPEST

   Expression

   Mammalian

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.27_eSOX17.2

   Plasmid

   #195499

   Purpose

   Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   Core distal endoderm enhancer region of SOX17 (SOX17 Human)
   

   Tags

   minP_luc2-hPEST

   Expression

   Mammalian

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIP-SFFV-mtagBFP-2A STING V155M 342STOP

    Plasmid

    #102594

    Purpose

    Lentivector to express STING V155M delta 342 and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    V155M delta 342

    Promoter

    SFFV

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223 SARS-CoV2 TEV-NSP5

    Plasmid

    #154404

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP5 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pLenti_EF1a_Flag_TP53_P278A

    Plasmid

    #183113

    Purpose

    Expresses flag-tagged p53 with P278A mutation in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 278 to alanine, see depositor com…

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223 SARS-CoV2 TEV-S

    Plasmid

    #154415

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of s…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Sept. 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLKO_FLAG.Ollas.VSVg_NGFR

    Plasmid

    #158324

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG.Ollas.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)

    Plasmid

    #209775

    Purpose

    CRISPRi for STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    dCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221 - myc ULK1 wt

    Plasmid

    #27623

    Depositor

    Reuben Shaw

    Article

    Egan et al Science. 2011 Jan 28;331(6016):456-61. d

    Insert

    mouse myc ULK1 (Myc Mouse)
    

    Use

    Gateway donr vector

    Tags

    myc

    Mutation

    Initial M from ULK1 removed. Silent mutation at A…

    Available Since

    Feb. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223 SARS-CoV2 TEV-NSP1

    Plasmid

    #154400

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    July 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pSF143-wArchon2-KGC-EGFP-ER2

    Plasmid

    #108430

    Purpose

    Codon-optimized Archon2 fluorescent voltage reporter in C.elegans expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    wArchon2-KGC-EGFP-ER2
    

    Expression

    Worm

    Promoter

    flp-18

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO_S.FLAG.VSVg_NGFR

    Plasmid

    #158345

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.FLAG.VSVg

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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