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Plasmid#229855PurposeTUBG2DepositorInsertTUBG2 (TUBG2 Human)
UseTagsFlagExpressionMammalianMutationRemoval of the internal TUBG2-EcoRI site by chang…PromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbmiR164e/h
Plasmid#231157PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbmiR164e/hDepositorInsertmobile gRNA targeting NbmiR164e/h
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_NbPDS
Plasmid#231147PurposeT-DNA encoding TRV2 with mobile gRNA targeting NbPDSDepositorInsertmobile gRNA targeting NbPDS
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmNeon Green-N1
Plasmid#202429PurposeMammalian expression vector containing mNeonGreenDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vh-Clover-CC-S2-mRuby2-Vt
Plasmid#233227PurposeVinculin FRET tension sensor with coiled-coil S2 domainDepositorInsertVh-Clover-CC-S2-mRuby2-Vt
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vh-Clover-CC-M2-mRuby2-Vt
Plasmid#233231PurposeVinculin FRET tension sensor with coiled-coil M2 domainDepositorInsertVh-Clover-CC-M2-mRuby2-Vt
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vh-Clover-CC-S1-mRuby2-Vt
Plasmid#233230PurposeVinculin FRET tension sensor with coiled-coil S1 domainDepositorInsertVh-Clover-CC-S1-mRuby2-Vt
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vh-Clover-CC-S0-mRuby2-Vt
Plasmid#233226PurposeVinculin FRET tension sensor with coiled-coil S0 domainDepositorInsertVh-Clover-CC-S0-mRuby2-Vt
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vh-Clover-CC-S4-mRuby2-Vt
Plasmid#233228PurposeVinculin FRET tension sensor with coiled-coil S4 domainDepositorInsertVh-Clover-CC-S4-mRuby2-Vt
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vh-Clover-CC-S7-mRuby2-Vt
Plasmid#233229PurposeVinculin FRET tension sensor with coiled-coil S7 domainDepositorInsertVh-Clover-CC-S7-mRuby2-Vt
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vh-Clover-CC-Mad-mRuby2-Vt
Plasmid#233232PurposeVinculin FRET tension sensor with coiled-coil Mad domainDepositorInsertVh-Clover-CC-Mad-mRuby2-Vt
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Vinculin-Clover-CC-S0-mRuby2
Plasmid#233233PurposeVinculin FRET tension sensor with coiled-coil S0 domain no vinculin force controlDepositorInsertVinculin-Clover-CC-S0-mRuby2
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEE844
Plasmid#231167PurposeT-DNA encoding TRV2 with PaqCI Recognition Sites and CcdbDepositorTypeEmpty backboneUseCloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Galt1-HT7-mGold
Plasmid#230027PurposeExpressing fluorescent HaloTag7 fusion protein in mammalian cells with localization in the Golgi apparatus.DepositorInsertGalt1-HaloTag7-mGold (B4GALT1 Human, Synthetic)
UseTagsHaloTag,mGoldExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
esgRNA_SlMIR164b
Plasmid#231153PurposeT-DNA encoding TRV2 with mobile gRNA targeting SlMIR164bDepositorInsertmobile gRNA targeting SlMIR164b
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ GFP-2xESP3I-IRES-mCherry
Plasmid#230996PurposeProtein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.DepositorTypeEmpty backboneUseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS19
Plasmid#229475PurposeExpresses RssB with E135A, E136A, & E137A mutationDepositorInsertRssB (E135A, E136A, E137A)
UseTagsExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMS21
Plasmid#229476PurposeExpresses RssB with E135A, E136A, & E137A mutation with His-tagDepositorInsertRssB (E135A, E136A, E137A)
UseTags10xHisExpressionBacterialMutationE135A, E136A, E137APromoterT7Available sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
UseTags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTYC1_pPromALB_enh5898_FireflyLuc
Plasmid#220285PurposeFirefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLucDepositorInsertmouse liver DNase hypersensitive site # 5898
UseLuciferaseTagsExpressionMutationPromoterminimal albumin promoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only