We narrowed to 17,261 results for: tes
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Plasmid#108861PurposeLbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutationsDepositorInsertLbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationG548R, K554V and Y558R, Cpf1 is rice codon optimi…Available sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H
Plasmid#108375PurposeFRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
ExpressionMammalianPromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTER GAMMA-TUBULIN shRNA Human
Plasmid#87955Purposereduced the expression of gamma-tubulin in human cellsDepositorInsertsh gamma-tubulin (TUBG1 Human)
TagsNo-tagExpressionMammalianMutationThe protein is a sh-gamma-tubulin resistant gene.…Available sinceOct. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mHDAC7-Unspliced (23-938) pEF6-V5 (MJS_017)
Plasmid#73266PurposeExpression of mouse HDAC7-U (23-938) in mammalian cells (V5 tag)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHDAC7 (Hdac7 Mouse)
TagsHis/V5ExpressionMammalianAvailable sinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
RAB14 3'UTR D1D2
Plasmid#61492Purposeluciferase reporter containing RAB14 3' UTR with both predicted MiR144 binding site deletedDepositorInsertRAB14 3' UTR (RAB14 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains position 837-1637 (numbering relative to…PromoterPGKAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLew90β-GW/T7/PARP SAS/luciferase/Aldolase 3′UTR
Plasmid#48157PurposeTransgenically expresses firefly luciferase protein in T. cruziDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertluciferase
UseLuciferase; T. cruzi expressionPromoterT7Available sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV HA hRB delta CDK + S249
Plasmid#58908Purposemammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restoredDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRb (RB1 Human)
TagsHAExpressionMammalianMutationdelta CDK* with S249 restoredPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLC-TIR-GFP
Plasmid#52736PurposeRetroviral vector expresses the lipid binding domain of PLCd1 fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsGFP and PLCd1 Lipid bindingExpressionMammalianMutationDeleted amino acids 1-85Available sinceJune 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
peGFPC2-CARKL
Plasmid#51748Purposeexpression of eGFP-fused mouse sedoheptulokinase (CARKL, SHPK)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSHPK (Shpk Mouse)
TagseGFPExpressionMammalianAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-CDC27 S379A
Plasmid#31662DepositorInsertcell division cycle 27 homolog S379A (CDC27 Human)
TagsM2ExpressionMammalianMutationchanged Serine 379 to AlanineAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
HNF4A_P2-135
Plasmid#31067DepositorInsertHNF4A P2 promoter (HNF4A Human)
UseLuciferaseExpressionMammalianMutation-135 to -1 of P2 HNF4A promoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 C540
Plasmid#25971DepositorInsertKinase suppressor of Ras 1 (Ksr1 Mouse)
TagsFlagExpressionMammalianMutationDeletes amino acids 1-539Available sinceAug. 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-eGFP-Ofd1-Myc-A80T
Plasmid#24579DepositorInsertOfd1 (Ofd1 Mouse)
TagsMyc and eGFPExpressionMammalianMutationAlanine 80 changed to threonine (codon GCA to ACA)Available sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Hmga2 ORFtr
Plasmid#14794DepositorInsertHmga2 ORFtr (Hmga2 Mouse)
ExpressionMammalianMutationTruncated ORF, but intact miRNA complementary sit…Available sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pB-3xFlag-dCas13-mTET2HxDCD
Plasmid#228235PurposeFor targeted tethering of an inactive mutant (HxD) of the catalytic domain of mouse TET2 using dCas13DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRExpressionMammalianMutationCD (cysteine-rich, dioxygenase domain) truncation…PromoterCAGAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_HyPer7RgDAAO
Plasmid#217746PurposeDonor vector for gateway cloning of HyPer7 DAAO, a fusion protein used to measure the uptake of D-amino acids across the plasma membrane.DepositorInsertHyPer7 D amino acid oxidase
TagsNuclear export signalExpressionMammalianMutationFused DAAO to the C-terminus of HyPer7 using a Gl…Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1 VAP-B KD/MD
Plasmid#226410PurposeExpression of human VAP-B (mutant K87D/M89D, unable to bind FFAT motifs) fused to mCherry in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVAP-B (VAPB Human)
TagsHA, T7-Xpress tag, and mCherryExpressionMammalianMutationK87D/M89D mutant, unable to bind FFAT motifsAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ-p21-HA(RxL)
Plasmid#215111PurposeExpresses p21-HA(RxL) in mammalian cells from an inducible lentiviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseLentiviralTagsHAExpressionMammalianMutationp21(RxL) denotes mutations: R19A, R20A, L21A, F22…PromoterCMV (Tet inducible)Available sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-SUMO1nc-PURO
Plasmid#208042PurposeEnables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycinDepositorInsertSUMO1nc (SUMO1 Human)
UseLentiviralTagsLow-affinity AvitagMutationQ94P Mutation near C-terminus suppresses cleavage…PromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSN840
Plasmid#184832PurposeExpresses human KIF5A(∆exon27) and human KLC1 in insect cellsDepositorUseCre/LoxTagsHis-FLAG and mScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterp10 and polyhedrinAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only