159,557 results

1. pCAGGS-myrGFP

   Plasmid

   #115502

   Purpose

   expression of myristoylated, plasma membrane targeted GFP

   Depositor

   Thomas Biederer

   Article

   Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

   Insert

   myristoylated GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179012

   Purpose

   Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)

   Plasmid

   #179008

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-NRAS-Q61K

   Plasmid

   #116444

   Purpose

   Lentiviral expression of NRAS Q61K

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Q61K

   Promoter

   Available since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJEC779

   Plasmid

   #234348

   Purpose

   Modular RENDR, GFP output, targets synthetic target

   Depositor

   James Chappell

   Article

   Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

   Insert

   Modular RENDR, GFP output
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEC780

   Plasmid

   #234349

   Purpose

   Expressed synthetic RNA target for RENDR

   Depositor

   James Chappell

   Article

   Gambill et al Nat Commun. 2023 Feb 1;14(1):543. doi: 1

   Insert

   Synthetic RNA target for RENDR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGD283

   Plasmid

   #129733

   Purpose

   Destination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.

   Depositor

   Rüdiger Simon

   Article

   Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV-EF1a-IRES-Neo

   Plasmid

   #85139

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 5'UTR CGG 99x FMR1-EGFP

   Plasmid

   #63091

   Purpose

   Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Actin-OptoNB

    Plasmid

    #159595

    Purpose

    Opto-Nanobody

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    ActinNB with AsLOV2 at loop 5 with 62-666
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEVOL-pylT-AznLRS

    Plasmid

    #172482

    Purpose

    For E.Coli expression, 306L309L348I384F411W

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

    Insert

    AznLRS and pylTcua
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHAGE-NRAS

    Plasmid

    #116767

    Purpose

    Lentiviral expression of NRAS

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

    Plasmid

    #171436

    Purpose

    Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    HaloTag N58-(GGS)X6-Lifeact
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX302 EGFP-V5

    Plasmid

    #141348

    Purpose

    Expresses V5 tagged GFP

    Depositor

    Kevin Janes

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCM66T: trimmed pCM66 backbone

    Plasmid

    #74738

    Purpose

    Small IncP-based broad host range replicating vector

    Depositor

    Mary Lidstrom

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLminP_Luc2P_RE47

    Plasmid

    #90385

    Purpose

    IFNB1 - Interferon beta gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFY

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-ataxin-3

    Plasmid

    #89975

    Purpose

    Expresses GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMV-Cas13X.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

    Plasmid

    #171379

    Purpose

    Expression vector for encoding a human codon-optimized Cas13X.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized Cas13X.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 8522-M01-663

    Plasmid

    #225660

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SORE6-mCMVp

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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