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pBabe-Sik-YF

Plasmid

#210723

Purpose

Construct for stable expression of constitutively active mouse Ptk6 (Sik) in mammalian cells.

Depositor

Angela Tyner

Article

Derry et al Mol Cell Biol. 2000 Aug;20(16):6114-26.

Insert

Protein tyrosine kinase 6 (Ptk6 Mouse)

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-PTK6-YF

Plasmid

#210715

Purpose

Construct for stable expression of constitutively active human PTK6 in mammalian cells.

Depositor

Angela Tyner

Article

Zheng et al Mol Cell Biol. 2010 Sep;30(17):4280-92.

Insert

Protein tyrosine kinase 6 (PTK6 Human)

Expression

Mammalian

Mutation

Tyrosine 447 changed to Phenylalanine

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide2 in pX458

Plasmid

#211536

Purpose

sgRNA-2 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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hLig4 guide1 in pX458

Plasmid

#211535

Purpose

sgRNA-1 against Lig4

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA hLig4 (LIG4 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-Puro

Plasmid

#211619

Purpose

Rhno1 delta TOPBP1 (T202A)

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

T202A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-FR-2Strep

Plasmid

#210312

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

2Strep

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-EGFP

Plasmid

#210318

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 fused with EGFP tag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 N-2Strep

Plasmid

#210376

Purpose

Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-COV-2 N (N SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PGUS

Plasmid

#207827

Purpose

FLAG-tagged PGUS driven by CMV promoter

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

PGUS

Use

Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:TDimer mVenus

Plasmid

#210520

Purpose

Expresses a tandem dimer of mVenus in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Article

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Tandem dimer mVenus

Use

Zebrafish expression, tol2 kit gateway-compatible

Promoter

Zebrafish insulin promoter

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:R198P mVenus

Plasmid

#210519

Purpose

Expresses mVenus-tagged R198P mutant of Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Article

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Mutation

R198P

Promoter

Zebrafish insulin promoter

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA ins:Apollo-NADP+ mVenus

Plasmid

#210518

Purpose

Expresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoter

Depositor

Jonathan Rocheleau

Article

Bui et al Sci Adv. 2023 Oct 6;9(40):eadi8317. doi:

Insert

Apollo-NADP+

Use

Zebrafish expression, tol2 kit gateway-compatible

Tags

mVenus

Promoter

Zebrafish insulin promoter

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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REEP6-2Strep

Plasmid

#210351

Purpose

Expresses REEP6 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP6 (REEP6 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pWR54

Plasmid

#203330

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
CCW12 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-fsr1

Plasmid

#179866

Purpose

Encodes the polyketide synthase Fsr1 from Fusarium vanettenii codon optimized for expression in Saccharomyces cerevisiae

Depositor

Jens Laurids Sørensen

Article

Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

Insert

Fusarubin polyketide synthase

Use

Synthetic Biology

Mutation

Codon optimized for expression in Saccharomyces c…

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-p62 var2 PB1mut

Plasmid

#204578

Purpose

Expresses 3xFLAG-p62 var2 PB1mut in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Expression

Mammalian

Mutation

K7A/D69A

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pWR24

Plasmid

#202771

Purpose

episomal bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coNAT
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWR16

Plasmid

#202711

Purpose

integrating bioluminescent reporter plasmid for S. stipitis

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Inserts

TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCBG
ADH2 terminator (S. stipitis)
URA3 and flanking sequence

Expression

Yeast

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_RV-B14_VP1

Plasmid

#202074

Purpose

Bacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

RV-B14 VP1 protein with Histag

Tags

6xHis-tag and GST

Expression

Bacterial

Promoter

tac

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMXSIP-Rab5B-HA

Plasmid

#204709

Purpose

expresses Rab5B-HA in mammalian cells

Depositor

William Kaelin

Article

Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

Insert

RAB5B (RAB5B Human)

Tags

HA

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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