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pHR- flag-Lag16-JAM-B

Plasmid

#205200

Purpose

This vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM JAM-B and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

Plasmid

#62908

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-NCAM-1

Plasmid

#205199

Purpose

This vector encodes of the synCAM (NCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM NCAM 1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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tetO7_Ef1a_SOX2

Plasmid

#18919

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

SOX2 (SOX2 Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pET28a(+)_beta2M (MHC Class I light)

Plasmid

#187357

Purpose

Bacterial expression and refolding of gp100 pMHC

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

beta2M (MHC Class I light)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-eLACCO1.1

Plasmid

#168790

Purpose

Astrocyte expression of fluorescent sensor for extracellular lactate

Depositor

Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

Insert

eLacco1.1

Use

AAV

Tags

ER export and GPI

Expression

Mammalian

Promoter

GFAP

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-TagBFP-W

Plasmid

#126687

Purpose

Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

TagBFP

Use

Lentiviral

Promoter

Ef1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag9-iCAM-1

Plasmid

#205209

Purpose

This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG9

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and Lag9 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat

Plasmid

#178883

Purpose

Vector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

U6-Nla repeat-BbsI-Nla repeat

Expression

Mammalian

Promoter

U6

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Human, Mouse)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

Plasmid

#62904

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD28 SNIPR

Plasmid

#188358

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP Rac2

Plasmid

#12193

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

Plasmid

#62951

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-LNGFR-FF3

Plasmid

#11666

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

Plasmid

#115209

Purpose

Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PbuCas13

Tags

SUMO

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

Plasmid

#62949

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMy-BirA-T2A-eGFP

Plasmid

#50565

Purpose

Retroviral vector encoding for GFP and the biotinylating enzyme BirA

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-Intb2

Plasmid

#205203

Purpose

This vector encodes of the synCAM (Intb2) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Intb2 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp4

Plasmid

#163588

Purpose

Expresses BMP-4 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp4 (Bmp4 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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