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pSTAP-seq_fly-ctrl

Plasmid

#86380

Purpose

Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

Depositor

Alexander Stark

Article

Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

Type

Empty backbone

Use

Stap-seq screening vector

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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PI(DM)-Rac1(WT)dc1.FLARE

Plasmid

#87589

Purpose

PI(DM): Photo-inhibitable/dark mutant C450A; dc1.FLARE: built based on GTPase FLARE dual chain sensor

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
PBD
Rac1

Tags

YPet and dTurq

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pQL269

Plasmid

#59802

Purpose

The univector plasmid-fusion system (UPS) uses Cre– lox site-specific recombination to fuse a plasmid containing the gene of interest and host vectors containing regulatory information.

Depositor

Stephen Elledge

Article

Liu et al Curr Biol. 1998 Dec 3;8(24):1300-9.

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Promoter

lambdaPR

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
RCAS (A) Caronte (CT#382)

Plasmid

#13862

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pBS Rootletin (Nigg pFL1(CW498))

Plasmid

#41168

Depositor

Erich Nigg

Article

Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. E

Insert

Rootletin (CROCC Human)

Expression

Bacterial

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
pIL2R FAK Y397F

Plasmid

#50525

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

IL-2R

Expression

Mammalian

Mutation

Y397F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pIRES2-eGFP-Ofd1-Myc-G139S

Plasmid

#24581

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Glycine 139 changed to serine (codon GGT to AGT)

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-S437R

Plasmid

#24582

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

Serine 437 changed to Arginine (codon AGT to CGT)

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-9b

Plasmid

#22710

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 9b (mir-9b primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-92b

Plasmid

#22709

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro-RNA-92b (mir-92b primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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