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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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179,317 results

Showing: 8721 - 8740 of 179317 results

  4. pKHR5

    Plasmid
    #74593
    Purpose
    an alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.
    Depositor
    David Grunwald
    Publication
    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:
    Insert
    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    Use
    Cre/Lox; Zebrafish expression
    Promoter
    alpha-crystallin
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74593-plasmid-map-sequence-id-140738

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  8. ADORA3-Tango

    Plasmid
    #66212
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADORA3 (ADORA3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66212-plasmid-map-sequence-id-485939

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  9. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Publication
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64217-plasmid-map-sequence-id-123057

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  10. pT7-V5-SBP-C1-HshnRNPM

    Plasmid
    #64924
    Purpose
    Expresses V5- and SBP-tagged hnRNPM in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    hnRNPM (HNRNPM Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64924-plasmid-map-sequence-id-109670

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  11. GFP-ESR1

    Plasmid
    #65211
    Purpose
    Full length mammalian expression vector for hESR1
    Depositor
    Ratna Vadlamudi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    esr1 (ESR1 Human)
    Tags
    AcGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65211-plasmid-map-sequence-id-422716

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  12. mCherry-CRY2-iSH2

    Plasmid
    #66839
    Purpose
    Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.
    Depositor
    Pietro De Camilli
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    iSH2
    Tags
    mCherry-CRY2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66839-plasmid-map-sequence-id-116784

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  18. pcDNA3-Lyn-ICUE3

    Plasmid
    #61623
    Purpose
    Plasma membrane-targeted FRET biosensor for monitoring cAMP
    Depositor
    Jin Zhang
    Publication
    DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn-ICUE3 (RAPGEF3 Human)
    Tags
    ECFP, N-terminal targeting sequence from Lyn kina…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61623-plasmid-map-sequence-id-100424

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  20. eGFPbait-E2A-KalTA4-pA donor vector

    Plasmid
    #61069
    Purpose
    for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)
    Depositor
    Filippo Del Bene
    Publication
    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    eGFPbait
    KalTA4
    Use
    Zebrafish expression
    Tags
    E2A
    Available since
    Feb. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61069-plasmid-map-sequence-id-99003

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Showing: 8721 - 8740 of 179317 results