We narrowed to 11,712 results for: NSI

1. dCas9-EWSR1_IDR-EGFP

   Plasmid

   #232762

   Purpose

   Expresses dCas9-EWSR1_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-EWSR1_IDR-EGFP (EWSR1 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-SS18_IDR-EGFP

   Plasmid

   #232763

   Purpose

   Expresses dCas9-SS18_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-SS18_IDR-EGFP (SS18 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-NONO1_IDR-EGFP

   Plasmid

   #232764

   Purpose

   Expresses dCas9-NONO1_IDR-EGFP

   Depositor

   Isaac Hilton

   Article

   Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

   Insert

   dCas9-NONO1_IDR-EGFP (NONO Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2T-CMV-EA-evoAPOBEC1max_NG-BlastR

   Plasmid

   #232721

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   evoAPOBEC1max H47E+S48A-nCas9(NG)-UGI-UGI
   

   Expression

   Mammalian

   Mutation

   within evoAPOBEC1: H47E+S48A; within Cas9 D10A

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLL5.0 shCavin1KDR-EGFP

   Plasmid

   #187254

   Purpose

   Lentiviral expression of shRNA targeting Cavin1 + reconstitution of mouse Cavin1 (Cavin1 rescue)

   Depositor

   Alpha Yap

   Article

   Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi

   Insert

   ShRNA for Cavin1 (Cavin1 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   U6, 5'LTR

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+ GFP-CDK5R1 degron (283-307)-IRES-mCherry

   Plasmid

   #231008

   Purpose

   Protein stability reporter construct for CDK5R1 consisting of aa 283-307 for transient overexpression in mammalian cells.

   Depositor

   Michael Rape

   Article

   Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

   Insert

   CDK5R1 aa283-307 (CDK5R1 Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 CHMP4B 156-224

   Plasmid

   #104596

   Purpose

   Expresses C-terminal 156-224 residues of CHMP4B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-7

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4B residues 156-224 (CHMP4B Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA528 CHMP1B 143-199

   Plasmid

   #108255

   Purpose

   Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP1B residues 143-199 (CHMP1B Human)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pICH86966::AtU6p::sgRNA:NbVPE1a/b

   Plasmid

   #223218

   Purpose

   Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

   Depositor

   Tsepo Tsekoa

   Article

   Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

   Insert

   NbVPE1a/b
   

   Use

   CRISPR and Synthetic Biology

   Tags

   None

   Expression

   Plant

   Promoter

   Arabidopsis U6 promoter

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSuper Retro GFP DGK zeta mouse

    Plasmid

    #224577

    Purpose

    Negative Control for downregulation of the expression of human DGK zeta in human cells

    Depositor

    Isabel Mérida

    Article

    Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

    Insert

    sh RNAi Diacylglycerol kinase zeta mouse (Dgkz Mouse)
    

    Use

    RNAi and Retroviral

    Promoter

    H1

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRX-IP-muGFP-hATG2A mLIR

    Plasmid

    #215506

    Purpose

    Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    Autophagy related 2A (ATG2A Human)
    

    Use

    Retroviral

    Tags

    muGFP

    Expression

    Mammalian

    Mutation

    P656R (natural variant with no functional relevan…

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAC-C Sars-CoV-2 S_L241-243del

    Plasmid

    #194841

    Purpose

    Sars-CoV-2 S_L241-243del in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    S_L241-243del (S Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    L241-243del

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pKG-GW1-hCIT K42E

    Plasmid

    #203168

    Purpose

    Yeast expression vector for human DHDDS K42E

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    DHDDS (DHDDS Human)
    

    Expression

    Yeast

    Mutation

    K42E

    Promoter

    TDH3

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Hb9-CD14

    Plasmid

    #204344

    Purpose

    Knock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human CD14 (CD14 Human)
    

    Expression

    Mammalian

    Promoter

    mouse Hb9 (Mnx1) 9kb promoter fragment

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528 CHMP4B, residues 156-224, no Cys

    Plasmid

    #180641

    Purpose

    Bacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4B (CHMP4B Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 156-224

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. hTln1-R1R2-17b

    Plasmid

    #191427

    Purpose

    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria

    Depositor

    Ben Goult

    Article

    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

    Insert

    Human Talin1 R1R2 domains (TLN1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    contains 17AA insertion from inclusion of exon 17b

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO

    Plasmid

    #195495

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA (SOX17 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti4/V5-DEST-LASSIE

    Plasmid

    #192105

    Purpose

    Lentiviral vector expressing long non-coding RNA LASSIE (linc00520)

    Depositor

    Reinier Boon

    Article

    Stanicek et al Commun Biol. 2020 May 26;3(1):265. doi:

    Insert

    LASSIE
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Coexpressed Free deadFPPS-NES

    Plasmid

    #182492

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    deadFPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX459-dMET

    Plasmid

    #176031

    Purpose

    A knock-out vector for dog MET

    Depositor

    Michiyuki Matsuda

    Article

    Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

    Insert

    A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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