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pCPmacroH2A.1-GS3

Plasmid

#204759

Purpose

Expression of Cas9 and human macroH2A.1

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

macroH2A1 (MACROH2A1 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B3

Plasmid

#103004

Purpose

non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B3 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.B2

Plasmid

#103003

Purpose

non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B2 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Stat1

Plasmid

#207227

Purpose

doxycycline-inducible expression of Mouse Stat1in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-STAT1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-LRRTM1

Plasmid

#82536

Purpose

Lentiviral vector for expressing HRP-LRRTM1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

LRRTM1 (LRRTM1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV N-term HA-FLAG puro BRD7

Plasmid

#41934

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Tags

HA-Flag

Expression

Mammalian

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
PET-Sniper SpCas9-NLS-6xHis

Plasmid

#207374

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 P.1

Plasmid

#173476

Purpose

Encodes SARS-CoV-2 P.1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

Insert

SARS-CoV-2 Spike Truncated to remove 18 amino acids

Expression

Mammalian

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-DAXX-D/E-His

Plasmid

#179619

Purpose

Express DAXX D/E region in bacteria

Depositor

Xiaolu Yang

Article

Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

Insert

death domain associated protein (DAXX Human)

Tags

GST, His, and TEV

Expression

Bacterial

Mutation

amino acids 414-505 of DAXX

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BTB+BACK)

Plasmid

#169723

Purpose

Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K, Y353E

Promoter

CMV

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCK341

Plasmid

#192640

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dSpRY has s…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ASB2

Plasmid

#207185

Purpose

doxycycline-inducible expression of Human ASB2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ASB2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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mH2A2-CT-GFP

Plasmid

#45171

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy2 (MACROH2A2, H2AFY2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-MTS-WT-c-Src-FLAG

Plasmid

#44652

Depositor

Yoshimi Homma

Article

Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

Insert

v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)

Tags

FLAG tag and mitochondria-targeting sequence (res…

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_linker

Plasmid

#211731

Purpose

Linker fragment for direct rescue of SARS-CoV-2, including 5' Promoter and 3' Termination signals and 100bp SARS-CoV-2 homology regions

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

Linker fragment SARS-CoV-2

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pAG491

Plasmid

#226747

Purpose

CRISPRi guide RNA against MTCH2 (dual guide)

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MTCH2 (MTCH2 Human)

Use

Lentiviral

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAG43

Plasmid

#226736

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

MICU1 (targeting sequence) (MICU1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-CMV-MAAP2

Plasmid

#218244

Purpose

1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.

Depositor

David Schaffer

Article

Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

Insert

Membrane-Associated Accessory Protein from Adeno-Associated Virus 2 driven by CMV promoter

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

Plasmid

#160608

Purpose

TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

P35s:dCas9:SRDX:tNOS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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