We narrowed to 9,085 results for: mel

1. pnYC-NvM-DmNot4_813-836opt-F818D_AF

   Plasmid

   #148695

   Purpose

   Bacterial Expression of DmNot4_813-836opt-F818D

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   DmNot4_813-836opt-F818D (CG31716 Synthetic)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnYC-NvM-DmNot4_813-836opt-L828E_AF

   Plasmid

   #148696

   Purpose

   Bacterial Expression of DmNot4_813-836opt-L828E

   Depositor

   Elisa Izaurralde

   Article

   Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

   Insert

   DmNot4_813-836opt-L828E (CG31716 Synthetic)
   

   Expression

   Bacterial

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. ppih.001.177.W133A

   Plasmid

   #137655

   Purpose

   Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

   Depositor

   Tara Davis, Melissa Jurica

   Article

   Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

   Insert

   ppih (PPIH Human)
   

   Tags

   GST-(His)6-thrombin

   Expression

   Bacterial

   Mutation

   001-177-W133A

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1137B=Hsp70Bb-Cas9

   Plasmid

   #153284

   Purpose

   The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Promoter

   Dmel Hsp70Bb

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pH6HTC_xtHUR-Halo

   Plasmid

   #175314

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   HUR (elavl1 X. tropicalis (frog))
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK528

   Plasmid

   #155388

   Purpose

   dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 511; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK434

   Plasmid

   #155378

   Purpose

   PphlF_A4NT, 1x cascade + [dCas9 + PA4-mVenus]. (Integration of 429; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   PphlF_A4NT in dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK422

   Plasmid

   #155373

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 412; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK423

   Plasmid

   #155374

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]. (Integration of 413; pOSIP)

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Available Since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHis-Laforin

    Plasmid

    #157989

    Purpose

    Expresses Laforin in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Choi et al J Kor Chem Soc, 2015; 59(5): 466-470.

    Insert

    Laforin (EPM2A Human)
    

    Tags

    His Tag

    Expression

    Bacterial

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcUW51-CgE

    Plasmid

    #13762

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

    Insert

    HSV-1 glycoprotein E
    

    Tags

    His

    Expression

    Insect

    Mutation

    C-terminal region of the HSV-1 gE (KOS strain ect…

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pJK520

    Plasmid

    #155386

    Purpose

    PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA1 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-MAP2K1-K57N

    Plasmid

    #116427

    Purpose

    Lentiviral expression of MAP2K1 K57N

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Mutation

    K57N

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-MAP2K1-P124S

    Plasmid

    #116428

    Purpose

    Lentiviral expression of MAP2K1 P124S

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Mutation

    P124S

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-MAP2K1-S222A

    Plasmid

    #116429

    Purpose

    Lentiviral expression of MAP2K1 S222A

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Mutation

    S222A

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-Hsp70-Cas9

    Plasmid

    #46294

    Purpose

    A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

    Depositor

    Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    codon optimized Cas9
    

    Use

    CRISPR

    Tags

    3x Flag

    Expression

    Insect

    Mutation

    Silent change from C to A at bp 834 of insert

    Promoter

    Hsp70

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHE-mEGFP-Map4

    Plasmid

    #105571

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    MAP4 (Map4 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYES260-sc.eEF2 K509A

    Plasmid

    #85120

    Purpose

    yeast expression of mutant EEF2

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K509A

    Promoter

    GAL1

    Available Since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a His6HuR

    Plasmid

    #135943

    Purpose

    Expresses HuR in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. CAHS2 (pBS0249)

    Plasmid

    #185139

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2
    

    Expression

    Mammalian

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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