Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,359 results for: NSI

Showing: 8741 - 8760 of 12359 results

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pCA528 CHMP5 159-222

Plasmid

#108259

Purpose

Expresses C-terminal 159-222 residues of CHMP5 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-10

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

CHMP5 residues 148-219 (CHMP5 Human)

Tags

His-SUMO

Expression

Bacterial

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 H143A

Plasmid

#224342

Purpose

Expresses human KDAC8 (HDAC8) H143A in E. coli

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 H143A (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Histidine 143 mutated to alanine

Promoter

T5

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC7 CD

Plasmid

#224345

Purpose

Expresses human KDAC7 (HDAC7) catalytic domain in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC7 (HDAC7 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Only includes residues 521-942 (catalytic domain).

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSuper Retro GFP DGK alfa human

Plasmid

#224578

Purpose

To stably downregulate the expression of human DGK alfa in human cells

Depositor

Isabel Mérida

Article

Torres-Ayuso et al Oncotarget. 2014 Oct 30;5(20):9710-26. d

Insert

sh RNAi Diacylglycerol kinase alfa human (DGKA Human)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-Rb1shRNA1

Plasmid

#220584

Purpose

To inducibly knockdown Rb1 expression

Depositor

Sean Lee

Article

Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

Insert

Rb1 shRNA1 (RB1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-Rb1shRNA2

Plasmid

#220585

Purpose

To inducibly knockdown Rb1 expression

Depositor

Sean Lee

Article

Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

Insert

Rb1 shRNA2 (RB1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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LT3GEPIR-Rb1shRNA3

Plasmid

#220586

Purpose

To inducibly knockdown Rb1 expression

Depositor

Sean Lee

Article

Magrath et al Cancer Res. 2024 May 2;84(9):1426-1442.

Insert

Rb1 shRNA3 (RB1 Human)

Use

Lentiviral

Promoter

TRE3G (TetOP) promoter

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pIRHD-TetR-GAL80-Rho1-GAL4

Plasmid

#218274

Purpose

Introduction of low-temperature-inducible GAL system, LowTempGAL v2.2

Depositor

Christopher Howard

Article

Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

Insert

gal80Arm1-pAgTEF1>KanMX4>tAgTEF1-pHAC1>UBI4>>DHFRP66L-TetR>NLS>TUP1>tABF1-pTEF2>Rho1C17R>>GAL4>tGAL4-pTEF1(TetO)>GAL80Arm2

Expression

Yeast

Mutation

DHFR (P66L), Rho1 (C17R)

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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MAC-C Sars-CoV-2 S_T1027I

Plasmid

#194850

Purpose

Sars-CoV-2 S_T1027I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_T1027I (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

T1027I

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_Y144del

Plasmid

#194854

Purpose

Sars-CoV-2 S_Y144del in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_Y144del (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

Y144del

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_E484K

Plasmid

#194836

Purpose

Sars-CoV-2 S_E484K in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_E484K (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

E484K

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_H655Y

Plasmid

#194837

Purpose

Sars-CoV-2 S_H655Y in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_H655Y (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

H655Y

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_K417N

Plasmid

#194838

Purpose

Sars-CoV-2 S_K417N in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_K417N (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

K417N

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_L18F

Plasmid

#194840

Purpose

Sars-CoV-2 S_L18F in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_L18F (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

L18F

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_L452R

Plasmid

#194842

Purpose

Sars-CoV-2 S_L452R in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_L452R (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

L452R

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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HA-C Sars-CoV-2 S_N501Y

Plasmid

#194843

Purpose

Sars-CoV-2 S_N501Y in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_N501Y (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

N501Y

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_P681H

Plasmid

#194845

Purpose

Sars-CoV-2 S_P681H in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_P681H (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P681H

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_Q52R

Plasmid

#194847

Purpose

Sars-CoV-2 S_Q52R in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_Q52R (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

Q52R

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 S_S982A

Plasmid

#194849

Purpose

Sars-CoV-2 S_S982A in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_S982A (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

S982A

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP5_K90R

Plasmid

#194819

Purpose

Sars-CoV-2 NSP5_K90R in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP5_K90R (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

K90R

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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