We narrowed to 11,712 results for: NSI

1. pCSmEn2n4ERT(#234)

   Plasmid

   #184082

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   6his-myc-chkEn2(n4)-ERT2
   

   Use

   Synthetic Biology

   Tags

   6his-Myc

   Expression

   Mammalian

   Mutation

   chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSmEn2SRERT(#231)

   Plasmid

   #184080

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   6his-myc-chkEn2(SR)-ERT2
   

   Use

   Synthetic Biology

   Tags

   6his-Myc

   Expression

   Mammalian

   Mutation

   chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT22UbhaEng2aE4PCh(#271)

   Plasmid

   #184085

   Purpose

   cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   3HA-ZfEng2a-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT22UbhaEng2bE4PCh(#272)

   Plasmid

   #184086

   Purpose

   cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   3HA-ZfEng2b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT22gCfpUbm5En2E4PCh(#251)

   Plasmid

   #184083

   Purpose

   cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-chkEn2-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT22Ubm5Eng2bE4PCh(#284)

   Plasmid

   #184088

   Purpose

   cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-ZfEng2b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT22UbHEn2E4PCh(#262)

   Plasmid

   #184084

   Purpose

   cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   3HA-chkEn2-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT22Ubm5Eng2aE4PCh(#283)

   Plasmid

   #184087

   Purpose

   cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-ZfEng2a-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCSmEn25EERT(#232)

   Plasmid

   #184081

   Purpose

   cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   6his-myc-chkEn2(5E)-ERT2
   

   Use

   Synthetic Biology

   Tags

   6his-Myc

   Expression

   Mammalian

   Mutation

   chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

   Promoter

   sCMV+SP6

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR221-ZNF385A

    Plasmid

    #101615

    Purpose

    Donor Vector containing ZNF385A transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF385A (ZNF385A Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-FOXD4L3

    Plasmid

    #101491

    Purpose

    Donor Vector containing FOXD4L3 transcription factor, part of the Human TFome Collection

    Depositor

    George Church

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    FOXD4L3 (FOXD4L3 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOmnibac_ZZ_linker_K490_GFP_SNAP

    Plasmid

    #159721

    Purpose

    Expresses kinesin-1 (amino acids 1-490) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (only amino acids 1-490), K490 (KIF5B Human)
    

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Only amino acids (1-490)

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnEA-vH_His-TEV-DSep1-msfGFP

    Plasmid

    #174494

    Purpose

    bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    Sep1 (Sep1 Fly)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dfnCas12a-KRAB_crRNA Td2

    Plasmid

    #176261

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-CMVtight-PLXNB2 iOE

    Plasmid

    #176849

    Purpose

    lentiviral vector for doxycycline-inducible PLXNB2 overexpression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hPLXNB2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Mutation

    full length PLXNB2

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV-hPLXNB2_PGK_Neo

    Plasmid

    #176848

    Purpose

    lentiviral vector for constitutive PLXNB2 overexpression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hPLXNB2 (PLXNB2 Human)
    

    Use

    Lentiviral

    Mutation

    full length PLXNB2

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO_S.C.FLAG_NGFR

    Plasmid

    #158332

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.C.FLAG

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO_S.C.NWS_NGFR

    Plasmid

    #158333

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.C.NWS

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO_S.C.HA_NGFR

    Plasmid

    #158334

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.C.HA

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO_S.C.AU1_NGFR

    Plasmid

    #158335

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    S.C.AU1

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available Since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge