We narrowed to 10,723 results for: SEC
-
Plasmid#160426PurposeExpression of c-luciferase from sp. Maristella sp. SVU (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-KHC-BZ
Plasmid#160427PurposeExpression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastPromoterAOXAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 MUT
Plasmid#110126PurposeExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activityDepositorInsertURH49 (DDX39A Human)
TagsFlagExpressionInsectMutation4 amino acid mutations (RSFS motif changed to KSL…PromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM33
Plasmid#154167PurposeAedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertRel2 (long isoform)
TagsFLAGExpressionInsectMutationC-terminal IκB domain deletedPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]
Plasmid#124068PurposeCRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)DepositorInserteb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
UseCRISPR and Cre/LoxExpressionInsectPromoterbhsp68Available SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-KD
Plasmid#114066Purposeprotein productionDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-WT
Plasmid#114065Purposeprotein productionDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS Jaws mCherry_tr
Plasmid#111550PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mCherryDepositorInsertJaws_mCherry_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104619PurposeExpresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4DepositorInsert3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
TagsFLAGExpressionInsectPromoterhsp70Available SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiex PKC - Peptide 14 mCit mCer
Plasmid#83565PurposeExpresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.DepositorInsertPKC alpha (PRKCA Human)
Tags10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…ExpressionBacterial and InsectMutationOnly catalytic domain of PKC (aa 335-672)PromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO h-PKD1 FLS (4127-4303) S4144D-Myc/His
Plasmid#83458PurposeHuman PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) and with a point mutant that disrupts the second binding domain (629)DepositorInsertPolycystin-1 (PKD1 Human)
Tags6xHis and MycExpressionMammalianMutationAmino-acids 4127-4303, S4144DAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
exon K-renilla
Plasmid#81007Purposeluciferase based mini-gene to report splicing at exon 25 in Drosophila paraylticDepositorInsertparalytic (para Fly)
TagsRenilla luciferaseExpressionInsectMutationspanning exon 24 to exon 26, termination codon in…PromoterP-AC5 (Actin 5C)Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 MCS-ZKSCAN1 548-847
Plasmid#69885PurposeExpresses a miniature version of the ZKSCAN1 circular RNA in DrosophilaDepositorAvailable SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA, Pbl-A DH domain
Plasmid#69794PurposePebble isoform A, DH domain, in P element-based pUAST vector for Gal4-regulated expression in Drosophila.DepositorInsertPebble DH domain
UseP element-based puast vector for gal4-regulated e…TagsHA-TagExpressionInsectMutationContains only the Pebble DH domainPromoterhsp70Available SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-W3083F
Plasmid#18076DepositorInsertDystrophin (DMD Human)
TagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …Available SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-W3083F/P3086A
Plasmid#18077DepositorInsertDystrophin (DMD Human)
TagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …Available SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-d-ZZ-W3083F
Plasmid#18078DepositorInsertDystrophin (DMD Human)
TagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …Available SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-d-ZZ-W3083F/P3086A
Plasmid#18079DepositorInsertDystrophin (DMD Human)
TagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …Available SinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
Neurl1Rm-Flag pBacPAK8-3
Plasmid#17326DepositorInsertNeuralized-like (Neurl1a Mouse)
TagsFLAGExpressionInsectMutationCys 521 changed to Ser Cys 524 changed to SerAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
Tags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only