161,641 results

1. pAAVS1-NDi-CRISPRi (Gen2)

   Plasmid

   #73498

   Purpose

   Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   dCas9-KRAB
   rtTA
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG and TRE3G

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-iGABASnFR2-WPRE

   Plasmid

   #218874

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   Synapsin

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA-3xHA-hTERT

   Plasmid

   #51637

   Purpose

   Mammalian expression of 3xHA tagged human TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   TERT (TERT Human)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-SauriCas9

   Plasmid

   #135964

   Purpose

   Expresses SauriCas9, and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-luciferase-P2A-Neo

   Plasmid

   #105621

   Purpose

   3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-Tol2 transposase

   Plasmid

   #158774

   Purpose

   Expression of Tol2 transposase

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   Tol2 tranposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Tol2 codon usage frequency well suitable for muri…

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-ERP2-(N-HA)KRAS_G12D

   Plasmid

   #201254

   Purpose

   Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

   Depositor

   Alexander Kleger

   Article

   Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

   Insert

   KRAS (KRAS Human)
   

   Use

   Piggybac transposon

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   changed Gly (G) 12 to Asp (D)

   Promoter

   TRE

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-mitoGFP

   Plasmid

   #44385

   Depositor

   Pantelis Tsoulfas

   Article

   Kitay et al Hum Mol Genet. 2013 Jan 24.

   Insert

   mitoGFP (COX8A Human)
   

   Use

   Lentiviral

   Tags

   Cox8 targeting sequence and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1MCS-2

   Plasmid

   #85168

   Purpose

   Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

   Depositor

   Kenneth Peterson

   Article

   Kovach et al Gene. 1995 Dec 1;166(1):175-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Constitutive (derepressed P-lac)

   Available since

   Nov. 8, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET21b Hot-PETase

    Plasmid

    #225761

    Purpose

    Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    Hot-PETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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