163,570 results

1. pET283xFlagPP7CPHis

   Plasmid

   #28174

   Depositor

   Kathleen Collins

   Insert

   PP7 coat protein optimized
   

   Use

   Tags

   6xhistidine and triple FLAG peptide

   Expression

   Bacterial

   Mutation

   C68A,C73A

   Promoter

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRB1017

   Plasmid

   #59936

   Purpose

   empty vector for gRNA cloning

   Depositor

   Andrew Fire

   Article

   Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available Since

   Sept. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGxRF3

   Plasmid

   #138384

   Purpose

   T2A-GAL4 knock-in cassette

   Depositor

   Shu Kondo

   Article

   Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

   Insert

   T2A-GAL4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF3347

   Plasmid

   #144823

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PITX1 (PITX1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EYFP-p65

   Plasmid

   #111192

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

   Insert

   RELA (RELA Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINJ

   Plasmid

   #26045

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   HisGST3C-lacZ
   

   Use

   Tags

   MAHHHHHH_GST_LEVLFQGT

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available Since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCWU6-xylR-E-luciferase

   Plasmid

   #190721

   Purpose

   fine tune expression of luciferase under the control of xylose- and theophylline- inducible system

   Depositor

   Chenggang Wu

   Article

   Bibek et al bioRxiv. 2023 Apr 24:2023.04.24.538132.

   Insert

   xylR-E-luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAB076i7

   Plasmid

   #79201

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-FLAG-hAR

    Plasmid

    #89080

    Purpose

    mammalian expression of FLAG-tagged human androgen receptor

    Depositor

    Elizabeth Wilson

    Article

    Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

    Insert

    hAR (AR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only