We narrowed to 21,797 results for: Sis
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Plasmid#138090PurposeLentiviral backbone for inducible expression of human GBP1 missing the reactive cysteine of the CaaX-box (GBP1-C589A)DepositorInserthuman GBP1 (GBP1 Human)
UseLentiviralTagsExpressionMammalianMutationcysteine 589 to alanine (C589A)Promoterminimal CMV promoter with heptamerized tet‐operat…Available sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabePuro-DNAJA4CDS
Plasmid#43799DepositorInsertDNAJA4 CDS (DNAJA4 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pLC-mCherry-MLKL
Plasmid#75167PurposeLentiCRISPR-mCherry with sgRNA targeting human MLKLDepositorInsertMLKL sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha2_SulR
Plasmid#186425Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoterDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF004-sgRNA-shuffle-in
Plasmid#104440PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF005DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterU6 promoterAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CIBN-EGFP-KRasCT
Plasmid#178527PurposeExpresses CIBN-EGFP-KRasCT in mammalian cellsDepositorInsertCIBN-EGFP-KRasCT
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Anc689-nCas9
Plasmid#163526PurposeMammalian CG-to-GC base editingDepositorInsertAnc689-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2-U3
Plasmid#173156PurposeSingle guide RNA cassette under U3 promoterDepositorInsertsgRNA cassette under U3 promoter
UseTagsExpressionPlantMutationPromoterpromoter region of the U3C snRNA gene (Marshallsa…Available sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127315PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST
Plasmid#127312PurposeExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-act1p
Plasmid#120233PurposeCarries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing act1p promoter and apramycin resistance gene
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_APOBEC1-UdgX-nCas9
Plasmid#163531PurposeMammalian CG-to-GC base editingDepositorInsertAPOBEC1-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLC-EGFP-RIP1
Plasmid#75163PurposeLentiCRISPR-EGFP with sgRNA targeting human RIPk1DepositorInsertRIPK1 sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS::AtHAP2-V5
Plasmid#132958PurposeExpress AtHAP2 full length protein in mammalian cellsDepositorUseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_EE-nCas9
Plasmid#163541PurposeMammalian CG-to-GC base editingDepositorInsertEE-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST_CAHS3_HYPDU (pBS0333)
Plasmid#185179PurposeFor the mammalian expression of the tardigrade protein CAHS3_HYPDU. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertCAHS3_HYPDU
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_APOE_HUMAN_E114I (pBS0839)
Plasmid#185274PurposeFor the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAPOE_HUMAN_E114I
UseTagsExpressionMammalianMutationE114IPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-miniSOG-VAMP2-citrine
Plasmid#50969PurposeAAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) constructDepositorInsertminiSOG-VAMP2-citrine (Vamp2 Arabdopsis thaliana, Mouse)
UseAAVTagscitrine and miniSOGExpressionMutationPromoterhuman synapsin promoterAvailable sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pST_AfrLEA3m (pBS0911)
Plasmid#185285PurposeFor the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertAfrLEA3m
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_PvLEA4_repeats_k3_26 (pBS0762)
Plasmid#185244PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertPvLEA4_repeats_k3_26
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-Nvec-codonopti-Cas9-2xNLS
Plasmid#141108PurposePlasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensisDepositorInsertNematostella codon optimized Cas9
UseUnspecifiedTagsExpressionMutationAll amino acids coded for by codons optimized for…PromoterT7Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPKm-196
Plasmid#90511PurposepcDNA3 - CMVmin PIF6-DBD, expressing PIF6-DBD under CMV promoterDepositorInsertPIF6-DBD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBI101-pGC1(D1)-GUS
Plasmid#49513PurposeExpresses beta-glucuronidase gene under the control of guard cell promoter (pGC1 (D1)) in plantDepositorInsertPromoter of GC1(D1) (AT1G22690 Mustard Weed)
UsePlant expressionTagsbeta-glucuronidaseExpressionMutationPromoterGC1 (At1g22690), 1141 bp upstream of the transcri…Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSW554 - pOp5:SP-mVenus-HDEL
Plasmid#116006Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST_SERF2_HUMAN (pBS0751)
Plasmid#185239PurposeFor the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertSERF2_HUMAN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA (pBS0310)
Plasmid#185169PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
cARA12-mRFP
Plasmid#181961PurposeARA12 protein tagged with mRFP, under control of TBA2 promoterDepositorInsertARA12 (ARA12 Mustard Weed)
UseTagsmRFPExpressionPlantMutationPromoterTBA2Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVL94 - pOp4:VND7
Plasmid#116016Purposedestination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-inductionDepositorInsertpOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
UseTagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-DNAJA4CDS
Plasmid#41849DepositorInsertDNAJA4 CDS (DNAJA4 Human)
UseLuciferaseTagsrenilla luciferaseExpressionMutationStart codon of DNAJA4 removedPromoterAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPKm- 226
Plasmid#90497PurposepcDNA3 - CMVmin - PIF3 - VPR expresses PIF3 and VPR transactivator domain , under CMV promoterDepositorInsertPIF3-VP64
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG
Plasmid#127308PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codonDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-CT
Plasmid#64063Purposemyc-tagged murine seipin (cytosolic C-terminus); lentiviral constructDepositorInsertbscl2 (Bscl2 Mouse)
UseLentiviralTagsmyc epitopeExpressionMammalianMutationContains Arg326 to Ser443PromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PEST
Plasmid#127316PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
psiCheck-ApoECDS
Plasmid#41848DepositorInsertApoE CDS (APOE Human)
UseLuciferaseTagsrenilla luciferaseExpressionMutationPromoterAvailable sinceFeb. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PEST
Plasmid#127318PurposeExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codonDepositorInsertnanoLuciferase
UseTags3xFLAG and CL1/PESTExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMyc-CMV1-huTLR4mut-C2141A
Plasmid#53527Purposeexpression of mutant TLR4 in mammalian cellsDepositorInsertTLR4 C2141A (TLR4 Human)
UseTagsMycExpressionMammalianMutationC2141APromoterCMVAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only