Addgene

Showing: 861 - 880 of 1192 results

Results per page:

Sort results by:

pET-NDH6

Plasmid

#105503

Purpose

Expression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tag

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

NDH6

Use

Tags

6xHis

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Use

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11a-dH3-dH4

Plasmid

#105502

Purpose

Co-expression of Drosophila histones H3 and H4

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH3-dH4

Use

Tags

Expression

Mutation

Promoter

Available since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-miniDi-P2A-mCherry

Plasmid

#204358

Purpose

AAV vector for miniDi expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDi-P2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

The third intracellular loop (ICL3) of hM4Di was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

Plasmid

#71396

Purpose

2Gi2R assay

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Inserts

2xGFP:NES
2xRFP:NLS

Use

Lentiviral

Tags

IRES

Expression

Mammalian

Mutation

Promoter

EF1alpha and IRES

Available since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-FLAG-GFP-Tmem192

Plasmid

#134630

Purpose

lysosomal marker

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-EF1a-tetOn-IRES-G418 (EtO)

Plasmid

#84776

Purpose

Second generation lenti tetracycline operator (on)

Depositor

Fred Gage

Article

Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

Insert

TET OPERATOR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

pTIGHT

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-miniDq-P2A-mCherry

Plasmid

#204357

Purpose

AAV vector for miniDq expression under the control of human synapsin promoter

Depositor

Masao Doi

Article

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-P2A-mCherry

Use

AAV

Tags

mCherry

Expression

Mutation

The third intracellular loop (ICL3) of hM3Dq was …

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM1-Tmem192-mRFP-3xHA

Plasmid

#134631

Purpose

bait for affinity-isolation of lysosomes

Depositor

Roberto Zoncu

Article

Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

Insert

TMEM192 (TMEM192 Human)

Use

Lentiviral

Tags

3xHA, TEV, and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p3HA-hMIB1

Plasmid

#33317

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

MIB1 (MIB1 Human)

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pPICZ-pre-Ost1-alphaf(I)-E2-Crimson

Plasmid

#117661

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-Ost1-alphaf(I)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3.1 hSCN5A_deltaSIV

Plasmid

#145771

Purpose

expresses human Nav1.5 lacking the SIV motif in mammalian cells

Depositor

Hugues Abriel

Article

Jespersen et al Biochem Biophys Res Commun. 2006 Oct 6;3

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

none

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Flt3-ITD-Y591F/Y919F

Plasmid

#74499

Purpose

Retroviral expression of FLT3 ITD-Y591F/Y919F

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

unknown

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCVL.SSA TLR (Sce)

Plasmid

#45576

Purpose

Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

5' iRFP arm
eGFP with I-Sce I TS
+3 mCherry
3' iRFP arm

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

embedded I-Sce I TS from 163-185bp, truncated 25 …

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p3F-Strep-mMALT1

Plasmid

#33315

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Malt1 (Malt1 Mouse)

Use

Tags

3xFLAG and StrepOne

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pPICZ-pre-pro-alphaf(I)-E2-Crimson

Plasmid

#117660

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf(I)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Topo Vglut2 in situ probe

Plasmid

#45639

Depositor

Jeffrey Macklis

Article

Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

Insert

Vglut2 in situ probe (Slc17a6 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains nt 2477-2993 of slc17a6 (Vglut2…

Promoter

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pFUPI hT5-S322-Cyp

Plasmid

#80282

Purpose

Expresses human TRIM5 CypA fusion

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion (TRIM5, PPIA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p3F-Strep-mBCL10

Plasmid

#33314

Depositor

Vanessa Redecke

Article

Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

Insert

Bcl10 (Bcl10 Mouse)

Use

Tags

3xFLAG and StrepOne

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only
pLL5.0 hCortKD-Flcortmut3YD EGFP

Plasmid

#187264

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

3YD (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge