We narrowed to 122,556 results for: CIL

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pcDNA3.1(+)-PM-CaNARi

Plasmid

#64850

Purpose

Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

Lyn-CaNARi

Tags

Cerulean, N-terminal targeting sequence from Lyn …

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-CaNAR2

Plasmid

#64729

Purpose

Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNAR2-NES

Tags

Cerulean3, Nuclear export signal (NES), and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-HM4Di-YFP

Plasmid

#209193

Purpose

Cre-dependent expression of Hm4Di fused to YFP; Chemogenetics

Depositor

Larry Zweifel

Article

Sanford et al Neuron. 2017 Jan 4;93(1):164-178. doi: 1

Insert

Hm4Di

Use

AAV

Tags

YFP

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pHSB04X

Plasmid

#117258

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019

Use

Other

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-HypaCAS9-pA

Plasmid

#131467

Purpose

Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

Insert

Codon optimized HypaCas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cyto-GCaMP3

Plasmid

#64853

Purpose

Cytosol-targeted version of the Ca2+ indicator GCaMP3.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

GCaMP3

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT70B

Plasmid

#218729

Purpose

Expresses N-terminally EGFP-tagged IFT70B in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Takei et al Biol Open. 2018 Apr 30;7(5). pii: bio.03

Insert

IFT70B (TTC30B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX6-Purg1

Plasmid

#39350

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

natMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HsRoquin1_AE

Plasmid

#148611

Purpose

Mammalian Expression of HsRoquin1

Depositor

Elisa Izaurralde

Article

Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

Insert

HsRoquin1 (RC3H1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAPB02

Plasmid

#65628

Purpose

A transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilis

Depositor

Pei-yuan Qian

Article

Li et al Sci Rep. 2015 Mar 24;5:9383. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAB035h

Plasmid

#79191

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Plac62 gIII, luxAB; Ptet rpoZ-HA4

Expression

Bacterial

Promoter

Plac62; Ptet

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAR357_SFP_pCDF-1b

Plasmid

#122851

Purpose

Co-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

Sfp

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Bst-LF

Plasmid

#145799

Purpose

Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

Depositor

Andrew Ellington

Article

Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

Insert

Bst-LF

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-ER-GCaMP3

Plasmid

#64854

Purpose

Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CYP450-GCaMP3

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPHB4 Sense

Plasmid

#60638

Purpose

Expresses the human EPHB4 exon 11/12 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Ephrin type B receptor 4 (EPHB4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pTK317

Plasmid

#59571

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(16) 2xK-turn Kozak EGFP attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pMA3287

Plasmid

#46883

Purpose

Lentiviral vector encoding Tet-inducible mitochondrially targeted Y147A mutant human Uracil-N-glycosylase

Depositor

Mikhail Alexeyev

Article

Shokolenko et al DNA Repair (Amst). 2013 Jul;12(7):488-99

Insert

Uracil-N-glycosylase, Y147A mutant (UNG Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Y147A

Promoter

TRE-Tight

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pMSR0

Plasmid

#161984

Purpose

Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

Depositor

Olga Soutourina

Article

Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 11, 2021

Availability

Academic Institutions and Nonprofits only

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