We narrowed to 2,433 results for: GCG

1. pU6-cGAS g2 Cas9-T2A-mCherry

   Plasmid

   #164253

   Purpose

   Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgcGAS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgDonor_lentiGuide

   Plasmid

   #184168

   Purpose

   LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Insert

   sgDonor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-sgNT3/Cre

   Plasmid

   #89654

   Purpose

   Expresses a non-targeting gRNA and Cre-recombinase

   Depositor

   Monte Winslow

   Article

   Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

   Insert

   non-targeting gRNA
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. ACE2 g1 + Cas9

   Plasmid

   #153011

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-NEAT1pr_v2

   Plasmid

   #97082

   Purpose

   Encodes an sgRNA that creates a DSB at the promoter of human NEAT1 gene

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   sgRNA NEAT1pr_v2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSFDuet1-sgRNA(siteT9)

   Plasmid

   #154832

   Purpose

   sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Insert

   sgRNA(siteT9)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-cGAS g3 Cas9-T2A-mCherry

   Plasmid

   #164254

   Purpose

   Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgcGAS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-blast shKGA

   Plasmid

   #110420

   Purpose

   shKGA, silence glutaminase KGA isoform, blasticidin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (RNA Pol III)

   Available since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. ULK1 KO sgRNA

   Plasmid

   #207561

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CCCGCCTGCGCCATGGAGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ACE2 g2

    Plasmid

    #153012

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

    Depositor

    Jason Sheltzer

    Insert

    ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. VPS35 shRNA1

    Plasmid

    #163858

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. miniCMV-tdTomado-tDeg

    Plasmid

    #185402

    Purpose

    tdTomado-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    tdTomado-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. PTEN-mut (negative control)

    Plasmid

    #86646

    Purpose

    expression of control shRNA

    Depositor

    Jaime Modiano

    Article

    Lin et al Mol Cancer. 2007 Feb 14;6:16.

    Insert

    PTEN (control)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. py100-sgRNA_hVEGFA

    Plasmid

    #198282

    Purpose

    Expression of SpCas9 guide RNA specific for human VEGFA locus

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    py100-sgRNA_hVEGFA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCY_gRNA_hPAX7-Pro_C5

    Plasmid

    #160456

    Purpose

    sgRNA targeting human PAX7 promoter

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7-Promoter_C5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCY_gRNA_hPAX7-Pro_C3

    Plasmid

    #160454

    Purpose

    sgRNA targeting human PAX7 promoter

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7-Promoter_C3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OA-1050J (GFP)

    Plasmid

    #133304

    Purpose

    express arrays of gRNA targeting GFP under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    GFP gRNA array
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6-3

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-U6-AasgRNA3.8

    Plasmid

    #121958

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b single chimeric gRNA, short version
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9-mCherry-HIST1H4C

    Plasmid

    #66944

    Purpose

    CRISPaint target selector HIST1H4C

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA HIST1H4C
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniCMV-EGFP-tDeg

    Plasmid

    #185400

    Purpose

    EGFP-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    EGFP-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge