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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS6-mVenus

Plasmid

#185806

Purpose

NDUFS6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS6 (NDUFS6 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA7-mVenus

Plasmid

#185647

Purpose

NDUFA7 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA7 (NDUFA7 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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MZB053

Plasmid

#217835

Purpose

Bacterial expression of human AP3B1 (1-677) and AP3M1 AP-3 core hemicomplex

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Inserts

AP3B1 (AP3B1 Human)
AP3M1 (AP3M1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Removed residues 678-1094 from AP3B1

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (450-517)-ULK1 (836-1050)-His

Plasmid

#222825

Purpose

Bacteria expression of MBP-ATG13 (450-517) and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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MBP-ATG13 (363-517)-ULK1 (836-1050)-His

Plasmid

#222824

Purpose

Bacteria expression of MBP-ATG13 C-terminal and ULK1 MIT domain fusion complex -6His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His and MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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His-GST-ATG13 (363-517)-ULK1 (836-1050)

Plasmid

#222822

Purpose

Bacteria expression of His-GST -ATG13 C-terminal and ULK1 MIT domain fusion complex

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

6His-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050)-msfGFP

Plasmid

#222821

Purpose

Mammalian expression of TwinStrep-Flag -ATG13 C-terminal and ULK1 MIT domain fusion complex fused with GFP-10His

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ATG13, ULK1 (ATG13, ULK1 Human)

Use

Tags

TwinStrep Flag and msfGFP-10His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA10-mVenus

Plasmid

#185648

Purpose

NDUFA10 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFB6-mVenus

Plasmid

#185652

Purpose

NDUFB6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFB6 (NDUFB6 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS3-mVenus

Plasmid

#185649

Purpose

NDUFS3 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS3 (NDUFS3 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA2-mVenus

Plasmid

#185645

Purpose

NDUFA2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA2 (NDUFA2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-NDUFA10-FKBP-HA

Plasmid

#185810

Purpose

NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Lentiviral

Tags

FKBP and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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lenti MS2-P65-HSF1_Hygro

Plasmid

#61426

Purpose

lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Insert

MS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

N55K in MS2

Promoter

EF1A

Available since

Dec. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

Plasmid

#216248

Purpose

HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human RANGAP1 (RANGAP1 Human)

Use

CRISPR and TALEN; Hdr template

Tags

miniIAA7.3xFlag.P2A.BSD

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCR2.1 Clover-LMNA Donor

Plasmid

#122508

Purpose

Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

Depositor

Graham Dellaire

Article

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)

Use

Homology repair template plasmid (donor plasmid)

Tags

Clover

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA.3.1.ADRB2-NP

Plasmid

#134376

Purpose

Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.

Depositor

Julien Hanson

Article

Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

Insert

ADRB2-NP (ADRB2 Human)

Use

Tags

Flag, natural peptide of nanoluciferase, and sign…

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-XRCC1-gRNA res-Neo

Plasmid

#176149

Purpose

XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

X-ray repair cross complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

Promoter

CMV

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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FLS2 FLS2_pECIA2

Plasmid

#115120

Purpose

Bait vector FLS2 FLS2_pECIA2 should be used with prey vector FLS2 FLS2_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G46330 (FLS2 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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FLS2 FLS2_pECIA14

Plasmid

#114920

Purpose

Prey vector FLS2 FLS2_pECIA14 should be used with bait vector FLS2 FLS2_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G46330 (FLS2 Mustard Weed)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge