We narrowed to 14,422 results for: nts

1. ATP6 DdCBE A1

   Plasmid

   #221946

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP6 DdCBE A2

   Plasmid

   #221947

   Purpose

   Expresses mitochondrial cytosine base editor arm in mammalian cells

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Insert

   COX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGK43

   Plasmid

   #222223

   Purpose

   Edits NPAS2 Gene.

   Depositor

   Seth Shipman

   Article

   Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a(+)-His(6)-AtPCO1(wt)

   Plasmid

   #191798

   Purpose

   Full length wt AtPCO1 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO1
   

   Tags

   6xHis, Thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-28a(+)-His(6)-AtPCO2(wt)

   Plasmid

   #191799

   Purpose

   Full length wt AtPCO2 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO2
   

   Tags

   6xHis, Thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a(+)-His(6)-AtPCO3(wt)

   Plasmid

   #191800

   Purpose

   Full length wt AtPCO3 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO3
   

   Tags

   6xHis, Thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLZRS-panTRK-CR1-EGFP

   Plasmid

   #187194

   Purpose

   To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3

   Depositor

   Pantelis Tsoulfas

   Article

   O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7.

   Insert

   CR1 pan-TRK receptor
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   mo-MLV or MMLV

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMYA5/pBSSK+

   Plasmid

   #97205

   Purpose

   CMYA5 (NM_153610, nt 12205 to 12279) in pBSSK+

   Depositor

   Rita Shiang

   Article

   Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

   Insert

   CMYA5 (CMYA5 Human)
   

   Use

   Unspecified

   Available Since

   July 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. CMYA5/pENTR3C

   Plasmid

   #97408

   Purpose

   CMYA5 (NM_153610, nt 12205 to 12279) in pENTR3C, Gateway Entry vector

   Depositor

   Rita Shiang

   Article

   Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

   Insert

   CMYA5 (CMYA5 Human)
   

   Use

   Unspecified

   Available Since

   July 18, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pCRII-Topo Tmtc4

    Plasmid

    #45869

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

    Insert

    TMTC4 in situ probe (Tmtc4 Mouse)
    

    Use

    In situ

    Mutation

    nt 1220-1798 on BC031368

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

11. MDH1-pre-let-7_PShairpin

    Plasmid

    #26537

    Depositor

    Chang-Zheng Chen

    Article

    Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

    Insert

    pre-let-7_PS hairpin
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    5 nt mutated to make pre-let-7 stem pairing perfe…

    Available Since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry-MS2coatProtein

    Plasmid

    #103832

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mCherry-MS2 coat protein (cp )
    

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TL1 DdCBE A1 (DddA6-C)

    Plasmid

    #221992

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. TL1 DdCBE T2 (DddA6-N)

    Plasmid

    #221993

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: Q1310R, S1330I, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJ548: pU6-SpuFz1_MS2-omegaRNA(75nt)-entry

    Plasmid

    #205269

    Purpose

    Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (BbsI guide entry site)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1 omegaRNA
    

    Expression

    Mammalian

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 7i sgRNA for 4-μHOM reporter

    Plasmid

    #113626

    Purpose

    sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    7i sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-dSa-VPR mini.-1X snRP1

    Plasmid

    #99687

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNL4-3 envFS eGFP Δvpu/env

    Plasmid

    #101345

    Purpose

    Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env until before the RRE

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Mutation

    delta vpu/Env

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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