We narrowed to 14,056 results for: ckb

Showing: 8781 - 8800 of 14056 results

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qTAG-N-Blast-moxGFP

Plasmid

#207677

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAC90-pmax-DEST

Plasmid

#48222

Purpose

Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

CRISPR; Gateway destination

Expression

Mammalian

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pST-Ki

Plasmid

#44563

Purpose

for integrative constitutive expression of mycobacterial proteins

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pBEVY-T

Plasmid

#51232

Purpose

bi-directional expression vector for yeast, constitutively active, TRP1 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3-LRF

Plasmid

#66936

Purpose

Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).

Depositor

Alvaro Monteiro

Article

Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV-40

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pCfB4586

Plasmid

#106124

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-3xFLAG

Plasmid

#207706

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCfB2189

Plasmid

#67532

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pQC

Plasmid

#221134

Purpose

Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator

Depositor

Daniel Daley

Article

Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102

Inserts

[PibpA-gfpASV]
[PcpxPmcherryASV] OPT

Expression

Bacterial

Promoter

cpxP and ibpA

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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Degron-KI SF3B1 donor vector

Plasmid

#65622

Purpose

Donor vector to generate Degron-KI DD-SF3B1

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

SF3B1 Degron-KI left and right HR arms

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
BlatCas9

Plasmid

#163792

Purpose

The plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.

Depositor

Yongming Wang

Article

Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pKAd5f11pES-PmeI

Plasmid

#122698

Purpose

To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Type

Empty backbone

Use

Adenoviral

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCH1 - pBait-empty

Plasmid

#174663

Purpose

arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

Depositor

Katherine Berry

Article

Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

Type

Empty backbone

Expression

Bacterial

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB2225

Plasmid

#67553

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Golden-Gate-Esp3I-Destination

Plasmid

#51278

Purpose

CAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3I

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Type

Empty backbone

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMP117

Plasmid

#207920

Purpose

GreenBraid destination vector pGBZ2 - PaqCI - Eco31I - spisPink- Eco31I - PaqCI

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Type

Empty backbone

Expression

Plant

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJH462

Plasmid

#172154

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Sumo-tagged pylBCD); AraC

Expression

Bacterial

Mutation

SUMO tag fused to N-terminus of PylB

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG005

Plasmid

#113980

Purpose

Level 1 high copy number vector for E. coli expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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