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pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101101

Purpose

Encodes dCas9(N) fused to transmembrane tether

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TMt-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA and Myc

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KMT2B_2

Plasmid

#101075

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KMT2B_1

Plasmid

#101074

Purpose

Encodes gRNA for 3' target of human KMT2B

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KMT2B (KMT2B Human)

Use

CRISPR

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_AHR_2

Plasmid

#101077

Purpose

Encodes gRNA for 3' target of human AHR

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against AHR (AHR Human)

Use

CRISPR

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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WT1 KI gRNA2

Plasmid

#92313

Purpose

CRISPR-GFP-gRNA for cutting WT1

Depositor

Sean Palecek

Publication

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WT1 (WT1 Human)

Tags

CRISPR GFP

Expression

Mammalian

Promoter

U6

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KDM1A_1

Plasmid

#86297

Purpose

Encodes gRNA for 3' target of human KDM1A

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA against KDM1A (KDM1A Human)

Use

CRISPR

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_DNMT3B

Plasmid

#72366

Purpose

Encodes gRNA for 3' target of human DNMT3B along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DNMT3B (DNMT3B Human)

Use

CRISPR

Available since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_2

Plasmid

#64255

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Publication

Savic et al Genome Res. 2015 Sep 9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Use

CRISPR

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330A_dCas9-1x7

Plasmid

#63601

Purpose

Expresses dCas9 and gRNA

Depositor

Takashi Yamamoto

Publication

Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized S. pyogenes dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

June 1, 2015

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x3

Plasmid

#58773

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHiUGE V5-epitope donor ORF1

Plasmid

#200378

Purpose

Tagging endogenously expressed proteins with V5 epitope, C-terminal

Depositor

Scott Soderling

Publication

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

V5-epitope

Expression

Mammalian

Mutation

NA

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE GS-gRNA (hSyn-Cre) backbone

Plasmid

#200368

Purpose

Backbone plasmid for cloning HiUGE GS-gRNA (with hSyn-Cre)

Depositor

Scott Soderling

Publication

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V3

Plasmid

#226956

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Publication

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag

Plasmid

#231591

Purpose

Express circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Publication

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-2xALFATag-10xSunTag

Expression

Mammalian

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Plasmid

#222993

Purpose

Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Publication

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Promoter

pCAG

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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Dual-CRISPR-Cas9-U6-H1 (pBP48)

Plasmid

#218932

Purpose

The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

Depositor

Baoxu Pang

Publication

Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

Type

Empty backbone

Use

CRISPR

Tags

2A-Puro and 3XHA

Expression

Mammalian

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMIA3-Olônne

Plasmid

#214447

Purpose

Expresses eSpCas9 and gRNA targeting Olônne-18 GSH

Depositor

Roger Foo

Publication

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_gRNA-ROSA26_hspCas9

Plasmid

#193310

Purpose

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Depositor

Simon Elsaesser

Publication

Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9 from S. pyogenes and U6 ROSA26 sgRNA (V2.0)

Use

CRISPR

Expression

Mammalian

Mutation

not applicable

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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