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pEntry_HCoV-OC43-Hemagglutinin-esterase

Plasmid

#168943

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Publication

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-Hemagglutinin-esterase (HE )

Use

Gateway Cloning

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP CL1

Plasmid

#182512

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP followed by the CL1 degron for constitutive gene expression knockdown.

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

CL1 Degron

Tags

GFP

Expression

Yeast

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCK671.306

Plasmid

#192641

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Publication

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->HA-mtC11L34

Plasmid

#162227

Purpose

Lentiviral vector for the expression of HA mtC11L34 in mammalian cells

Depositor

Wendell Lim

Publication

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mtC11L34

Use

Lentiviral

Expression

Mammalian

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMVS-V1

Plasmid

#183094

Purpose

Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

Depositor

Lars Angenent, Bastian Molitor

Publication

Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

Inserts

pME2001
thermostable neomycin phosphotransferase gene

Use

Bacterial propagation and conjugative

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTU387

Plasmid

#178981

Purpose

pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

Depositor

Stan Brouns

Publication

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

CRISPR array from Candidatus Scalindua brodae

Expression

Bacterial

Promoter

T7

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

Plasmid

#172066

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

Depositor

Daichi Kamiyama

Publication

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pVG49

Plasmid

#164707

Purpose

yEVenus under the control of GAL1 promoter

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

GAL1pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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AlphaC

Plasmid

#124525

Purpose

pColE1_AlphaC

Depositor

Koen Venken

Publication

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDB026a

Plasmid

#109260

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Publication

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);

Expression

Bacterial

Mutation

amber codon at position 29 gIII

Promoter

Ppsp; PproK

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miRFP

Plasmid

#108410

Purpose

Monomeric near-infrared fluorescent protein miRFP in bacterial expression plasmid

Depositor

Edward Boyden

Publication

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Expression

Bacterial

Promoter

araB

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET26-CNTnw

Plasmid

#100163

Purpose

recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

Depositor

Seok-Yong Lee

Publication

Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

Insert

Concentrative nucleoside transporter

Tags

MBP-His

Expression

Bacterial

Promoter

T7

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTlpA-Wasabi-NF

Plasmid

#86100

Purpose

Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A and S71T, G73A

Promoter

TlpA and TlpA, LacI

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLacI241-Wasabi

Plasmid

#86102

Purpose

mWasabi reporter gene gated by temperature-sensitive LacI A241T

Depositor

Mikhail Shapiro

Publication

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

LacIts
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

A241T

Promoter

T7A1O4/O3 and pLacI

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBCC155

Plasmid

#74995

Purpose

CFP-based BiFC vector for transient expression of CC (155-238) in plants

Depositor

Yutaka Kodama, Masamitsu Wada

Publication

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

CFP (155–239)/CC155

Expression

Plant

Promoter

CaMV35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-GST-CD-ATL (Cbx7)

Plasmid

#82527

Purpose

Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 7 (CBX7 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1–84

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-mKate2-myc no-pA

Plasmid

#80812

Purpose

myc epitope fused to C-terminus of mKate2 without pA

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mKateHA p3E

Expression

Mammalian

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHUK

Plasmid

#64183

Purpose

harbours marker genes HIS3, URA3 and LYS2

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHM

Plasmid

#64173

Purpose

harbours marker genes HIS3 and MET17

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2048

Plasmid

#79656

Purpose

ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Publication

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple259-EmGFP WPRE

Use

AAV

Promoter

PAX6 MiniPromoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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