162,309 results

1. pKM512

   Plasmid

   #140192

   Purpose

   For curing of Orbit-integrated plasmids and associated drug marker

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Inserts

   zeocin resistance gene
   Bxb1gp47 (directionality factor)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cloned in gp47 directinality factor in place of R…

   Promoter

   EM7 and Ptet

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. phOct4-EGFP

   Plasmid

   #38776

   Depositor

   Wei Cui

   Article

   Gerrard et al Stem Cells. 2005;23(1):124-33.

   Insert

   Oct-4 promoter (POU5F1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   human OCT4

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. UbC-blasticidine-P2A-TETon-3G transactivator

   Plasmid

   #200191

   Purpose

   A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.

   Depositor

   Jacco van Rheenen

   Article

   Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:

   Insert

   blasticidine - P2A - TETon-3G transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   UbC

   Available since

   Aug. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_EF1a_flagLaM4-Notch-Gal4VP64

   Plasmid

   #216669

   Purpose

   Expresses anti-mCherry synNotch Gal4VP64

   Depositor

   Leonardo Morsut

   Insert

   anti-mCherry synNotch Gal4VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-GFP

   Plasmid

   #160697

   Purpose

   Expresses GFP in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-FLuc+GFPd2

   Plasmid

   #127190

   Purpose

   Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)

   Depositor

   Jordan Green

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-NES-KIRIN1

   Plasmid

   #107416

   Purpose

   Mammalian cell expression plasmid for potassium ion raitometric indicator KIRIN1 with nuclear exclusion sequence

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Nuclear exclusion sequence (NES) (LALKLAGLDIGS)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin and U6

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. spNW50

   Plasmid

   #173486

   Purpose

   Expresses spNW50 in Bl21

   Depositor

   Huan Bao

   Article

   Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

   Insert

   spNW50
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3-N90-beta-catenin

    Plasmid

    #31785

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-90- Constitutitvely Active

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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