We narrowed to 13,211 results for: CAR

1. pLKO_AU1.NWS.Ollas_mCherry-NLS

   Plasmid

   #178227

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.NWS.Ollas and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO_AU1.HSV.V5_mCherry-NLS

   Plasmid

   #178226

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HSV.V5 and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO_AU1.HSV.NWS_mCherry-NLS

   Plasmid

   #178222

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HSV.NWS and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO_AU1.HA.NWS_mCherry-NLS

   Plasmid

   #178217

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   AU1.HA.NWS and Nuclear Localization Signal

   Promoter

   eF1a

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-uTEV1-MAVS-mTA6

   Plasmid

   #171004

   Purpose

   HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

   Plasmid

   #166855

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K683E, H685E, K686E

   Promoter

   pCu

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

   Plasmid

   #166854

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   F641E, I645E

   Promoter

   pCu

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

   Plasmid

   #166853

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   F641G, I645G

   Promoter

   pCu

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mWasabi-ADAP1

   Plasmid

   #163906

   Purpose

   Full length mouse ADAP1 with N terminal fluorescent tag for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   ADAP1 (Adap1 Mouse)
   

   Tags

   mWasabi fluorescent tag

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

    Plasmid

    #160609

    Purpose

    TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:MS2-VP64:Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_Donor/Reporter-COL4A3

    Plasmid

    #130279

    Purpose

    COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    mCherry-eGFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_COL4A3_self-cleavingCas9

    Plasmid

    #130281

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A3 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV_COL4A5_self-cleavingCas9

    Plasmid

    #130282

    Purpose

    Expresses an spCas9 under the control of a CMV promoter; The spCas9 is flanked by COL4A5 sgRNA targets to induce its self-cleaving and inactivation

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    spCas9
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. prCOrev

    Plasmid

    #153961

    Purpose

    Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the opposite direction
    

    Use

    Retroviral; A reporter plasmid detecting dna reco…

    Tags

    NLS-ZF

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    LTR

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. prCO

    Plasmid

    #153960

    Purpose

    Used to detect crossover-type DNA recombination events elicited by iso-positional nicking of two homologous DNA fragments (recombination analogous to Holliday's model)

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    5' and 3' EGFP fragments sharing a 386-bp sequence, separated by a NeoR gene cassette and arranged in the same direction
    

    Use

    Retroviral; A reporter plasmid detecting dna reco…

    Tags

    NLS-ZF

    Expression

    Mammalian

    Mutation

    No mutation

    Promoter

    LTR

    Available Since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid

    #136656

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and hU6

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid

    #136655

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and hU6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid

    #136653

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and hU6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_SLC25A53

    Plasmid

    #132043

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A53 (SLC25A53 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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