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Showing: 8841 - 8860 of 8882 results
  1. pTRE3G-FLAG-human cGASdeltaN-HA

    Plasmid
    #186848
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    FLAG and HA
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

    Plasmid
    #223166
    Purpose
    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE
    Depositor
    Insert
    KRAB-MECP2 (MECP2 Human)
    Use
    AAV and CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Encodes aa 204-310 (MECP2)
    Promoter
    EF1a
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pGBW-m4046911

    Plasmid
    #145695
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 helicase
    Insert
    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. pGBW-m4046851

    Plasmid
    #145614
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 helicase
    Insert
    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
  6. pVZ_PcoaT_SQE1_MRN1

    Plasmid
    #103858
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and MRN1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Insert
    marneral synthase 1 (MRN1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized2nd amino acid …
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pVZ_PcoaT_SQE1_CAS1

    Plasmid
    #103856
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and CAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Insert
    cycloartenol synthase 1 (CAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available since
    Jan. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pVGI-1084-C77G

    Plasmid
    #261732
    Purpose
    Expresses CD45.1 with the C77G mutation for the purpose of investigating the functional consequences of this splicing mutation.
    Depositor
    Insert
    Murine CD45.1 Minigene (Ptprc Mouse)
    Expression
    Mammalian
    Mutation
    C77G mutation (cysteine to glycine at amino acid …
    Promoter
    human LFA
    Available since
    Oct. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
  9. pET28a 6xHis-HaloTag-TEVc-4xtrTUBE

    Plasmid
    #254154
    Purpose
    Bacterial expression of 4xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Insert
    4xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    HaloTag, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
  10. pET28a 6xHis-HaloTag-TEVc-6xtrTUBE

    Plasmid
    #254156
    Purpose
    Bacterial expression of 6xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Insert
    6xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    6xHis, HaloTag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
  11. pTRE3G-FLAG-human cGASdeltaN-GFP

    Plasmid
    #186879
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Insert
    Human cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal GFP fusion (Adamts1 Human, Synthetic)
    Use
    Retroviral
    Tags
    FLAG and GFP
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 8841 - 8860 of 8882 results