We narrowed to 17,075 results for: sta
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Plasmid#124806PurposeHDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMurine IgA
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Scara3-IRES-BFP
Plasmid#117265PurposeRetroviral overexpression of Scara3DepositorInsertScara3 (Scara3 Mouse)
UseRetroviralExpressionMammalianMutationSee depositor comments below.PromoterLTRAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
Tagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN2-C911
Plasmid#125786Purposea seed-matched control for pRSITEP-puro-shWRN2DepositorInsertshWRN2 (WRN Human)
UseRNAiAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG
Plasmid#127322PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgWRN1
Plasmid#125771Purposeconstitutive expression of a guide RNA targeting human WRNDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgWRN1 (WRN Human)
UseCRISPRAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003-sgWRN2
Plasmid#125772Purposeconstitutive expression of a guide RNA targeting human WRNDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsgWRN2 (WRN Human)
UseCRISPRAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
PacrAB-acrAB-rfp + PmutS-mutS-yfp
Plasmid#121443Purposeplasmid expressing translational fusions of AcrAB and MutSDepositorInsertsPmutS-mutS-yfp
PacrAB-acrAB-rfp
UseSynthetic Biology; Pbb vectorExpressionBacterialPromoterPacrAB and PmutSAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-191084-gp160
Plasmid#123234PurposeMammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolateDepositorInsertHIV-1 (191084 B7-19) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-H031gp160
Plasmid#123272PurposeMammalian expression plasmid for Env from the H031 HIV-1 isolateDepositorInsertHIV-1 (H031) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mCitrine
Plasmid#117994PurposeExpression of mCitrine controlled by the CaMV 35S promoterDepositorInsertmCitrine
UsePlant/bacteria binary vectorExpressionPlantPromoterCauliflower mosaic virus 35SAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B
Plasmid#108883PurposeMultisite gateway vector for 5' tagging with the human histone H2B sequence (for nuclear localisation)DepositorInsertH2B (human amino acid sequence but different codon usage) (H2BC21 Synthetic, Human)
UseGateway CloningMutationhuman amino acid sequence but different codon usa…Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPB1-CTD14
Plasmid#112822PurposepSMF2 with 14 CTD repeatsDepositorInsertRPB1 (RPO21 Budding Yeast)
ExpressionYeastMutationCTD repeats have identical sequence, deleted repe…PromoterNative promoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBV163
Plasmid#108364PurposeUsed to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.DepositorInsertKnock-in Cg TDH3 promoter in Cg YAP1 gene
UseReplace c.glabrata yap1 promoter with c. glabrata…PromoterTDH3Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG233
Plasmid#105838PurposeExpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tagDepositorPublicationInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 233PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA TAG186
Plasmid#105837PurposeExpresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tagDepositorPublicationInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
Tags6x his tagExpressionBacterialMutationTAG stop codon at site 186PromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD765 HMGCR 3'UTR
Plasmid#105766PurposeEncodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHMGCR 3'UTR (HMGCR Human)
UseLuciferaseMutationNoneAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMGF157
Plasmid#96972Purposeish1-mCherry insertion cassette with Hygro resistance geneDepositorInsertish1 (ish1 Fission Yeast)
TagsGST and mCherryExpressionBacterialMutationish1 C-terminal domain fused with mCherry + hygro…PromoterN/AAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN1
Plasmid#96979PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in plants.DepositorAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMDC43-SEIPIN3
Plasmid#96978PurposeExpress GFP-tagged Arabidopsis SEIPIN3 gene in plants.DepositorAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by