We narrowed to 19,276 results for: Tat

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pEYC1A

Plasmid

#127758

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in convergent orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGex4T1-JET

Plasmid

#136245

Purpose

Protein expression of Jetlag

Depositor

Aziz Sancar

Article

Ozturk et al J Biol Chem. 2014 Feb 21;289(8):4634-42.

Insert

Jetlag

Tags

GST

Expression

Bacterial

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD051-sgCh2-2

Plasmid

#125775

Purpose

constitutive expression of a guide RNA targeting an intergenic region of human chromosome 2 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgCh2-2

Use

CRISPR

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA

Plasmid

#124624

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MS2_adRNA_RAB7A

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-clbK

Plasmid

#114152

Purpose

Expresses ClbK

Depositor

Emily Balskus

Article

Zha et al ACS Chem Biol. 2016 May 20;11(5):1287-95

Insert

clbK

Tags

6xhis-Thrombin

Expression

Bacterial

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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T5-sgRNA

Plasmid

#113655

Purpose

sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

Depositor

Jeffrey Barrick

Article

Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

Insert

promoter T5 and sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

T5

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDB326

Plasmid

#113670

Purpose

expresses Ulp1_R2 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R2

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCD294.9

Plasmid

#113319

Purpose

dCas9, PCP-SoxS_R93A, J109 scRNA.b1

Depositor

Jesse Zalatan

Article

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

dCas9, PCP-SoxS_R93A, J109 scRNA.b1_PP7

Use

CRISPR

Expression

Bacterial

Mutation

R93A

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pKER-mRuby2

Plasmid

#85977

Purpose

Expresses mRuby2 from the EF1a promoter in mammalian cells

Depositor

Michele Calos

Article

Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

Insert

mRuby2

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPR137B_WT_V5

Plasmid

#83017

Purpose

Gateway Donor vector containing GPR137B, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

GPR137B (GPR137B Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAP2_WT_V5

Plasmid

#83000

Purpose

Gateway Donor vector containing STAP2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STAP2 (STAP2 Human)

Use

Gateway Cloning

Mutation

D93N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB31_WT_V5

Plasmid

#82993

Purpose

Gateway Donor vector containing RAB31, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RAB31 (RAB31 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FAIM_WT_V5

Plasmid

#82930

Purpose

Gateway Donor vector containing FAIM, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

FAIM (FAIM Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMAL-c2G

Plasmid

#75290

Purpose

Expresses proteins in the cytoplasm as fusions to maltose-binding protein

Depositor

Paul Riggs

Article

Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

Type

Empty backbone

Tags

maltose-binding protein

Expression

Bacterial

Promoter

tac

Available Since

July 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pJL1604

Plasmid

#78194

Purpose

Ce cha-1 expression in motor neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

unc-3

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
plentiCRISPR-sgMDH1

Plasmid

#72875

Purpose

The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

Depositor

David Sabatini

Article

Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

Insert

sgMDH1

Use

Lentiviral

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
circRAJ31-Toehold_mutant

Plasmid

#69930

Purpose

Expresses a toehold mutant of the circRAJ31 riboregulator encased in a permuted intron exon ribozyme, which circularises the riboregulator. This mutant does not activate cisRAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

plTet-circRAJ31_toeholdmutant

Use

Synthetic Biology

Mutation

4 bases mutated in toehold of riboregulator

Promoter

PltetO1-2

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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MP-S3

Plasmid

#69692

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 d80-AAG(Y127I-H136L)

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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MP-P7

Plasmid

#69666

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR polB

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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MP-Q6

Plasmid

#69676

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA ugi APOBEC1

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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