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Showing: 8901 - 8920 of 19451 results

  1. pLCHKO_Ptbp_ex8_3

    Plasmid
    #155083
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155083-plasmid-map-sequence-id-308532

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  11. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Publication
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Synthetic, Human)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  12. pKE13-ISceI-fabp10a:CreERT2

    Plasmid
    #198242
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter driving tamoxifen-inducible Cre recombinase
    Depositor
    Didier Stainier
    Publication
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:Cre recombinase (Cre) fused to a mutant estrogen ligand-binding domain (ERT2), polyA (fabp10a Zebrafish)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198242-plasmid-map-sequence-id-393628

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  13. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid
    #217767
    Purpose
    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)
    Depositor
    Paula Nunes-Hasler
    Publication
    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do
    Insert
    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    Use
    Protein purification (6xhis tag, tev site, sumo3 …
    Tags
    sfGFP
    Expression
    Bacterial
    Mutation
    CA3 domain aa 317-400
    Promoter
    T7 promoter
    Available since
    June 10, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217767-plasmid-map-sequence-id-435788

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  14. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid
    #207356
    Purpose
    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.
    Depositor
    Marianne Carlon
    Publication
    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d
    Insert
    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    Use
    Lentiviral
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    207356-plasmid-map-sequence-id-408417

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  15. pCAX-SCLM

    Plasmid
    #216757
    Purpose
    For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter
    Depositor
    George Augustine
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SuperClomeleon
    Expression
    Mammalian
    Mutation
    S30R and 11 AA deletion in the Cerulean CFP moiet…
    Promoter
    CAG (cytomegalovirus-chicken beta-actin-rabbit be…
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216757-plasmid-map-sequence-id-429395

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  16. NSP4-WT-Flag

    Plasmid
    #210339
    Purpose
    Expresses SARS-CoV-2 NSP4 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Publication
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210339-plasmid-map-sequence-id-418188

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  17. pcDNA 3.1-nsp7-FLAG

    Plasmid
    #201027
    Purpose
    Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.
    Depositor
    Sharon Rozovsky
    Publication
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    8xHis and FLAG
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    201027-plasmid-map-sequence-id-394458

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  18. pCRISPR Human CASP9 -1

    Plasmid
    #198419
    Purpose
    Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein
    Depositor
    Hannah Rabinowich
    Publication
    Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Human CASP9 Specific gRNA (CASP9 Human)
    Use
    CRISPR
    Tags
    Cas9/Orange Fluorescent Protein Reporter
    Expression
    Mammalian
    Promoter
    U6; CMV
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198419-plasmid-map-sequence-id-390148

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  19. pOUPc-UL148-HA

    Plasmid
    #179343
    Purpose
    All-in-one "tet-on" 3G lentiviral vector plasmid encoding human cytomegalovirus UL148 ER resident glycoprotein with a C-terminal HA tag fused to its cytoplasmic tail
    Depositor
    Jeremy Kamil
    Publication
    Siddiquey et al J Virol. 2018 Sep 26;92(20). pii: JVI.00
    Insert
    human cytomegalovirus UL148 fused to HA epitope
    Use
    Lentiviral and Synthetic Biology; Hiv-1 based rep…
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    insert matches UL148 from strain TB40/E
    Promoter
    Tet responsive element 3G
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    179343-plasmid-map-sequence-id-355065

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  20. pB80-hsKIF5B(1-560)Rigor-L-2xHA

    Plasmid
    #193718
    Purpose
    Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules
    Depositor
    Lukas Kapitein
    Publication
    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Synthetic, Human)
    Tags
    2xHA
    Expression
    Mammalian
    Mutation
    hsKIF5B(aa1-560), RIGOR: G234A
    Promoter
    Chicken beta-actin
    Available since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193718-plasmid-map-sequence-id-383730

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Showing: 8901 - 8920 of 19451 results