We narrowed to 12,975 results for: lic

1. pGFP-mTet1s_K852R pc3914

   Plasmid

   #201704

   Purpose

   Expresses of GFP-tagged mTet1s_K852R in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s-K852R (Tet1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 852 mutated to arginine

   Promoter

   CAG

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGFP-mTet1s_K852E pc3915

   Plasmid

   #201705

   Purpose

   Expresses of GFP-tagged mTet1s_K852E in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s-K852E (Tet1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Lysine 852 mutated to glutamate

   Promoter

   CAG

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JO-pH720-SARS-CoV-2-S-tail-WT

   Plasmid

   #218453

   Purpose

   Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

   Depositor

   Syed Saif Hasan

   Article

   Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

   Insert

   Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)
   

   Tags

   eXact

   Expression

   Bacterial

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCBE3-HF1

   Plasmid

   #218156

   Purpose

   This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-HF1
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCBE3-HF1-Hypa

   Plasmid

   #218158

   Purpose

   This plasmid harbors the base editor SCBE3-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-HF1-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N497A…

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSCBE3-Hypa

   Plasmid

   #218157

   Purpose

   This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   scbe3-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutations in the portion of SpCas9 : D10A / N692A…

   Promoter

   rpsL promoter

   Available Since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV2_CAG_oROS-HT(C199S)_WPRE

   Plasmid

   #216415

   Purpose

   Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT(C199S)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

   Plasmid

   #208966

   Purpose

   Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…

   Promoter

   CMV and T7

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

   Plasmid

   #208973

   Purpose

   Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   ePhi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPR v2-sgABI2-1

    Plasmid

    #210139

    Purpose

    knock out ABI2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Abl interactor 2 (ABI2 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiCRISPR v2-sgCYFIP1-1

    Plasmid

    #210137

    Purpose

    knock out CYFIP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. lentiCRISPR v2-sgABI2-2

    Plasmid

    #210140

    Purpose

    knock out ABI2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Abl interactor 2 (ABI2 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. lentiCRISPR v2-sgHSPC300-1

    Plasmid

    #210141

    Purpose

    knock out HSPC300 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Protein BRICK1 (BRK1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. lentiCRISPR v2-sgHSPC300-2

    Plasmid

    #210142

    Purpose

    knock out HSPC300 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Protein BRICK1 (BRK1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiCRISPR v2-sgCYFIP1-2

    Plasmid

    #210138

    Purpose

    knock out CYFIP1 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

    Insert

    Cytoplasmic FMR1-interacting protein 1 (CYFIP1 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cav2.2 W391A pcDNA3

    Plasmid

    #206085

    Purpose

    expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

    Depositor

    Annette Dolphin

    Article

    Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Expression

    Mammalian

    Mutation

    W391A

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

    Plasmid

    #196878

    Purpose

    Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2-N-gTBD
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-mCherry-KATNA1, V55D

    Plasmid

    #180651

    Purpose

    Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Has V55D mutation. Dox-inducible. Internal ID: WISP20-48.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRNA resistant to: GGACAGCACUCCCUUGAAA

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 CHMP4C, residues 156-233, no Cys

    Plasmid

    #180642

    Purpose

    Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C (CHMP4C Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 316-366

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 IST1, residues 315-360, no Cys

    Plasmid

    #180643

    Purpose

    Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 315-360

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge