We narrowed to 15,736 results for: LEA

1. mCitrine-LaminB1-10

   Plasmid

   #56562

   Purpose

   Localization: Nuclear Envelope, Excitation: 516, Emission: 529

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mCitrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 23, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. RFXANK (3UXG)

   Plasmid

   #36902

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RFXANK (RFXANK Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Available Since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

3. NR1D2

   Plasmid

   #25186

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NR1D2_07 (NR1D2 Human)
   

   Use

   Baculovirus expression

   Tags

   His

   Promoter

   polyhedrin

   Available Since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pResQ shFEN3 3XF-FEN1 D181A

   Plasmid

   #17753

   Depositor

   Sheila Stewart

   Insert

   Flap Endonuclease I (FEN1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Triple Flag

   Expression

   Mammalian

   Mutation

   D181A

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

5. Dronpa-LaminB1-10

   Plasmid

   #57282

   Purpose

   Localization: Nuclear Envelope, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   Dronpa

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pHL 1477

   Plasmid

   #69787

   Purpose

   KanR with FRT sites + ptet::OmpC::T7RNAP::T1 terminator + ColE

   Depositor

   Han Lim

   Article

   Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

   Insert

   T7RNAP
   

   Use

   Synthetic Biology

   Tags

   OmpC leader

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   Nov. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pRSETA_ionKE

   Plasmid

   #172925

   Purpose

   Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

   Depositor

   Arnold Boersma

   Article

   Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

   Insert

   IonicStrengthSensorKE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5J39 (TDRKH)

   Plasmid

   #79523

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TDRD2 (TDRKH Human)
   

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   June 16, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. mAmetrine-LaminB1-10

   Plasmid

   #56547

   Purpose

   Localization: Nuclear Envelope, Excitation: 406, Emission: 526

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mAmetrine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. UHRF1

    Plasmid

    #25359

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubh12.672.793 (UHRF1 Human)
    

    Tags

    His with thrombin cleavage site

    Expression

    Bacterial

    Mutation

    amino acid residues 672-793.

    Available Since

    Dec. 21, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

11. YPet-LaminB1-10

    Plasmid

    #56635

    Purpose

    Localization: Nuclear Envelope, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. USP37

    Plasmid

    #36898

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    USP37 (USP37 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 4-125 only

    Available Since

    June 21, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

13. 61 pLIB_N-Strep_C_FKBP

    Plasmid

    #160310

    Purpose

    N-term 3C cleavable 2xStrepII, C-term FKBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII and FKBP

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 60 pLIB_HIS_MYC

    Plasmid

    #160309

    Purpose

    N-term 3C cleavable His-Myc tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    12xHis and 6xMyc

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. OTU04

    Plasmid

    #28157

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    OTU domain containing 7A (OTUD7A Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    UBA-like domain only

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

16. tdEos-CENPA-C-22

    Plasmid

    #57597

    Purpose

    Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    CENPA (CENPA Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Please see Depositor Comments

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCVL RipTAL(Nd154C63) SFFV Age-Xho/Xba-Sal linkers

    Plasmid

    #50626

    Purpose

    pRipTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.

    Depositor

    Andrew Scharenberg

    Type

    Empty backbone

    Use

    Lentiviral and TALEN

    Expression

    Mammalian

    Available Since

    May 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. BC-GPRC

    Plasmid

    #44199

    Depositor

    Lei Xu

    Insert

    human GPR56 C-terminal fragment (ADGRG1 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    C-terminal fragment of GPR56 starting at amino a…

    Promoter

    LTR

    Available Since

    May 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CTraf

    Plasmid

    #27808

    Depositor

    Steven Vogel

    Article

    Thaler et al Biophys J. 2005 Oct . 89(4):2736-49.

    Insert

    Cerulean Traf (TANK Human)
    

    Expression

    Mammalian

    Available Since

    June 23, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pET22b-EC12-129/138

    Plasmid

    #47926

    Purpose

    Expresses E-cadherin EC12 with C9A, K129C/D138C mutations in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Tags

    6xHis and TEV Cleavage

    Expression

    Bacterial

    Mutation

    Mutations C9A, K129C, D138C

    Promoter

    T7

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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