159,258 results

1. pAAV-Ef1a-Con/Fon oG

   Plasmid

   #131778

   Purpose

   INTRSECT 2.0

   Depositor

   Karl Deisseroth

   Article

   Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

   Insert

   rabies glycoprotein - oG
   

   Use

   AAV; Intrsect

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)

   Plasmid

   #149722

   Purpose

   Lentiviral expression of crRNA with GFP and a neomycin resistance gene

   Depositor

   Junwei Shi

   Article

   Gier et al Nat Commun. 2020 Jul 13;11(1):3455. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTag-gal4-VP16-B-actin

   Plasmid

   #117817

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   gal4/VP16
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAW63.YY1.FKBP.knock-in.BFP

   Plasmid

   #104371

   Purpose

   For knocking in FKBP F36V P2A BFP

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   FKBP12(F36V) -P2A-BFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUGW-APPwt-T2A-mCherry

   Plasmid

   #190803

   Purpose

   Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry

   Depositor

   Ronald Hart

   Insert

   APP (APP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ubi C

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. L4440_BioBrick-hif-1-sgRNA-A

   Plasmid

   #177784

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (targeting C. elegans hif-1 promoter)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   hif-1 (hif-1 Nematode)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF3060

   Plasmid

   #144536

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTALdNC-AAVS1_T1

   Plasmid

   #80495

   Purpose

   AAVS1 TALEN expression vector (Left)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Left)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTALdNC-AAVS1_T2

   Plasmid

   #80496

   Purpose

   AAVS1 TALEN expression vector (Right)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Right)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-Ubiquitin-KO

    Plasmid

    #17603

    Purpose

    Mammalian expression of HA tagged ubiquitin carrying zero lysines (K0)

    Depositor

    Ted Dawson

    Article

    Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    KO: all lysines mutated to arginines

    Promoter

    Available since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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