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PDE4B-Sensor
Plasmid#208929Purposein vitro JNK-Sensor for luminescent detection of JNK-activityDepositorInsertPDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXPHM3-NB-D6
Plasmid#167989Purposeexpression of a nanobody against rat PepT2 as a n terminal MBP fusionDepositorInsertNanobodyD6
UseTagsHis-MBPExpressionBacterialMutationPromoterpBADAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
Plasmid#81185PurposeExpresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5cDepositorInsertsCas5c
Cas8c
Cas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X-ORF24-NTD
Plasmid#138465PurposeExpresses MBP-tagged ORF24-NTD in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p300dAIL KAT LIC 1M
Plasmid#233588PurposeBacterial expression of p300 KAT enzyme with truncated autoinhibitory loopDepositorInsertp300dAIL KAT (EP300 Human)
UseTags6xHis MBPExpressionBacterialMutationdeleted amino acids 1523-1554PromoterT7Available sinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
hnRNPAB X2-RBD
Plasmid#216516PurposeExpresses MBP-tagged RNA binding domain of hnRNPAB X2DepositorInserthnRNPAB X2-RBD
UseTags6xHis tags-MBPExpressionBacterialMutationdeleted amino acids 2-64 and 226-304PromoterAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSD118A
Plasmid#218140PurposeExpresses AviTag-6xHis-MBP-TEV-SD0(ZF2-3) for protein purification from E. coliDepositorInsertSD0_ZF2-3
UseTagsAviTag-6xHis-MBP-TEV-(GGGGS)2ExpressionMutationPromoterAvailable sinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
RC(14-3-3ε)-LgBit
Plasmid#208926PurposeRecognition domain for in vitro RSK-activity sensorDepositorInsert14-3-3ε (YWHAQ Human)
UseTagsHISx6, LgBit, and MBPExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVSp1 pET24b
Plasmid#128088PurposeBacterial expression vector for NaVSp1 bacterial sodium channel. It expresses for HIStag-MBP-3C-channel proteinDepositorInsertNaVSp1p
UseTags3C Cleavage, His Tag, and MBPExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pMal-Strep
Plasmid#209345PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMal-HA
Plasmid#209346PurposeProtein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagIIDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGTM_YR375_SUMO_Protease_001
Plasmid#190063PurposeExpresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusionDepositorInsertULP1_YEAST (ULP1 Budding Yeast)
UseTags8X His-MBP (Maltose Binding Protein)ExpressionBacterialMutationConstruct contains residues 347 – 621PromoterT7 lacAvailable sinceJan. 30, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
HMA-APOBEC1-Nb
Plasmid#226012PurposeFor expression of His-MBP-APOBEC1-Nanobody protein in E.coliDepositorInsertAPOBEC1 (Apobec1 Rat)
UseTagsHis-MBP and antiRabbit-nanobodyExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
spCas9-NLS-MAV
Plasmid#124212PurposeSpCas9-NLS 17 amino acid linker to monoavidin (SpCas9-NLS-MAV)DepositorInsertcas9
UseCRISPRTagsHis6, MBP, TEV Cleavage site, and two copies of S…ExpressionBacterialMutationPromoterT7Available sinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KEAP1_KELCH
Plasmid#178476PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKEAP1_KELCH (KEAP1 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHMTc-FBF-2 (121-632)
Plasmid#218664PurposeExpression of MBP:6xHIS:FBF-2(121-632)DepositorInsertfbf-2 (fbf-2 Nematode)
UseTagsMBP-6xHISExpressionBacterialMutationamino acids 121-632 onlyPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK2097
Plasmid#218665PurposeExpression of MBP:6xHIS:FBF-2(Y479A, 121-632)DepositorInsertfbf-2 (fbf-2 Nematode)
UseTagsMBP-6xHISExpressionBacterialMutationamino acids 121-632 only, with Y479A mutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KPNA4_Arm
Plasmid#178478PurposeBacterial expression of human domain with His-tag and MBP tagDepositorInsertKPNA4_Arm (KPNA4 Synthetic, Human)
UseTagsHis-MBPExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X-ORF24-NTD-3L_A
Plasmid#138466PurposeExpresses MBP-tagged ORF24-NTD 3L_A in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201; amino acids L73-75 mutated to …PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only
