Addgene

We narrowed to 19,489 results for: ACE

Showing: 881 - 900 of 19489 results

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mCherry-FDD

Plasmid

#80629

Purpose

Fluorescent destablizing domain for regulating protein stability and fluorescence

Depositor

Thomas Wandless

Article

Navarro et al ACS Chem Biol. 2016 Jun 6.

Insert

UnaG

Use

Retroviral

Tags

HA-mcherry-linker

Expression

Mutation

mutations A36V, R136G

Promoter

LTR

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCJ162

Plasmid

#175055

Purpose

lox66-72 flanked gentamycin resistance cassette integrates into aquI integration site

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

lox66::gmR::lox71

Use

Unspecified

Tags

Expression

Mutation

Promoter

n/a

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBP_sgRNA

Plasmid

#190128

Purpose

Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ionRE

Plasmid

#172932

Purpose

Ionic strength FRET sensor with mCerulean3 and mCitrine for mammalian cell expression.

Depositor

Arnold Boersma

Article

Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

Insert

IonicStrengthSensorRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMB1-A

Plasmid

#190114

Purpose

Level 1 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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DVC_p15A_FG

Plasmid

#217590

Purpose

Destination vector with chloramphenicol resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-LSSmCherry1(-13)

Plasmid

#219415

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

LSSmCherry1(-13)

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

K137E, K199E

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMB2a

Plasmid

#190120

Purpose

Level 2 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDD103

Plasmid

#119887

Purpose

Constitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pConstitutive_mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pPAP004

Plasmid

#160911

Purpose

E.coli/ P.pastoris shuttle plasmid for episomal expression in P.pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

Insert

lacZ selection cassette

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pROP-PP-GFP

Plasmid

#122135

Purpose

GFP Reporter plasmid for B-Rex contain perfect operator sequence

Depositor

Srivatsan Raman

Article

Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDrBB2

Plasmid

#18946

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

hygromycin gene driven by the copia promoter, phiC31 attB

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pDD87

Plasmid

#119892

Purpose

The pLPD03031 promoter from Rhodococcus opacus PD630 expressing GFP+ on the pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pLPD03031_GFP+

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - mSRSF6

Plasmid

#120420

Purpose

overexpression of mouse Srsf6

Depositor

Andreas Neueder, Gillian Bates

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (Srsf6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV immediate/early

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pACM4

Plasmid

#49797

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Article

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Use

Tags

S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pACYCDUET-StrepSUMO-DaPFOR

Plasmid

#217819

Purpose

Expresses DaPFOR in E. coli

Depositor

Pamela Silver

Article

Ng et al ACS Chem Biol. 2024 Feb 16;19(2):380-391

Insert

pACYCDUET-StrepSUMO-DaPFOR

Use

Tags

StrepSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAB202

Plasmid

#101674

Purpose

Opto-T7RNAP(563)

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP(563)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMK-Prrn (N. tabacum)

Plasmid

#123490

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

Prrn (N. tabacum)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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YCe3736_HC_Amp_ccdB_receiver vector

Plasmid

#100637

Purpose

EMMA toolkit receiver vector

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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