We narrowed to 13,363 results for: BASE

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pYPQ269C2

Plasmid

#164716

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible haid-cbe_v04 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ269A2

Plasmid

#164714

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v04 entry…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265

Plasmid

#164712

Purpose

Gateway entry clone (attL1 & attR5) rAPOBEC1-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

rAPOBEC1-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible rapobec1-cbe_v01 entry…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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BE3-hA3A-Y130F

Plasmid

#132945

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

BE3-hA3A(Y130F)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

BE3-hA3A(Y130F)

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ269D

Plasmid

#164717

Purpose

Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

zCas9(D10A)-PmCDA1-UGI-T2A-MS2-UGI)

Use

CRISPR; Gateway compatible pmcda1-cbe_v04 entry c…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TGmiR-Gb2

Plasmid

#25746

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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ABE8.13-d

Plasmid

#136297

Purpose

expresses ABE8.13-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK-Neo-TmiR-Gb2

Plasmid

#25744

Purpose

Control lentiviral vector with Tet-based inducible expression of mouse G beta 2 miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Gb2 (Gnb2 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G12

Plasmid

#25740

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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AID-C12*-n'Cas9-BE4max

Plasmid

#216730

Purpose

Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

Depositor

Rahul Kohli

Article

Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

Insert

AID-C12*-n'Cas9-BE4max (AICDA Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TadA-N46L-NG-CBE

Plasmid

#227419

Purpose

C-to-T base editing in mammalian cells

Depositor

Yang Sun

Article

Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

Insert

TadA-N46L-nSpCas9-NG-2xUGI (AICDA Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pnCas9-miniSdd3-PBE

Plasmid

#204857

Purpose

For cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ265C

Plasmid

#164715

Purpose

Gateway entry clone (attL1 & attR5) hAID-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

hAID-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible haid-cbe_v01 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265E1

Plasmid

#164718

Purpose

Gateway entry clone (attL1 & attR5) A3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible a3a-cbe_v01 entry clone

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ265E3

Plasmid

#164720

Purpose

Gateway entry clone (attL1 & attR5) eA3A-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

eA3A-zCas9(D10A)-UGI

Use

CRISPR; Gateway compatible ea3a-cbe_v01 entry clo…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A T31A-nCas9

Plasmid

#163538

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A T31A-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)eA3A T31A

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-nCas9

Plasmid

#163564

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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BE3-YE1

Plasmid

#132943

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

BE3-(W90Y-R126E)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

BE3-(W90Y-R126E)

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSOUP

Plasmid

#165419

Purpose

The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

Depositor

Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

Article

Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

Insert

Rep A

Use

Helper plasmid required for replication for all p…

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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ATeam1.03-nD/nA/pcDNA3

Plasmid

#51958

Purpose

mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

Depositor

Takeharu Nagai

Article

Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

Insert

ATeam1.03-nD/nA

Expression

Mammalian

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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