We narrowed to 2,256 results for: D10

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attB-GFP-Tet1d834-1363-Poly(A)

Plasmid

#65560

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Tet1 (Tet1 Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

del(834-1363)

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Human Wild-type ASAP1

Plasmid

#235212

Purpose

Expresses Wild-Type ASAP1 in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al Proc Natl Acad Sci U S A. 2018 May 1;115

Insert

ASAP1 (ASAP1 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

Plasmid

#185911

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8e-nSpG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

Plasmid

#140003

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

P2A-EGFP

Expression

Mammalian

Mutation

nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-APOBEC1

Plasmid

#209039

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

APOBEC1-nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

Plasmid

#185916

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-evoCDA1

Plasmid

#209042

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

evoCDA1-nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-APOBEC3A

Plasmid

#209040

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

APOBEC3A-nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-NoCBE

Plasmid

#209038

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-nSpCas9-BEACON2

Plasmid

#209041

Purpose

Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

BEACON2-nSpCas9

Use

CRISPR and Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

D10A

Promoter

Tight TRE promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EDAR-COMP5AP-AviTag-9xHis

Plasmid

#157119

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EDAR (EDAR Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_PEX3_WT

Plasmid

#81779

Purpose

Gateway Donor vector containing PEX3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PEX3 (PEX3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

Plasmid

#197504

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-ABE8e-NRCH-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_PEX3_p.G173E

Plasmid

#81623

Purpose

Gateway Donor vector containing PEX3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PEX3 (PEX3 Human)

Use

Gateway entry vector

Mutation

G173E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

Plasmid

#197506

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

Plasmid

#201820

Purpose

An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging

Depositor

Christopher Richie

Article

Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

Insert

hChR2(H134R)-V5-ER-LBD (ChRERa)

Use

AAV

Promoter

SYN1

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

Plasmid

#197505

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

Promoter

CMV and T7

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A16_STOP

Plasmid

#161145

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A16 (SLC25A16 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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nCas9-UGI control for Target-AID (pRZ1047)

Plasmid

#131299

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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KHC065 pBABE pU6 BRD2 K1-2C

Plasmid

#231712

Purpose

KHC065 (pBABE pU6 BRD2 K1-2C), gRNA and cas9D10A mammalian expression vector for CRISPR mediated cutting of BRD2 at the N-terminus, use with KHC064

Depositor

Alessio Ciulli

Article

Bond et al J Med Chem. 2021 Oct 28;64(20):15477-155

Insert

gRNA for Brd2 and Cas9D10A (BRD2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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