We narrowed to 8,310 results for: Dos

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pLenti-EML4-ALK variant 1

Plasmid

#183828

Purpose

Express EML4-ALK fusion variant 1 (E13;A20) in mammalian cells

Depositor

Aaron Hata

Article

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-13 and ALK exon…

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-DDR2

Plasmid

#23897

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DDR2 (DDR2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pUAST-pHuji-GINKO2

Plasmid

#177117

Purpose

Genetically encoded green fluorescent potassium ion indicator GINKO2 and red fluorescent pH indicator pHuji fusion

Depositor

Robert Campbell

Article

Wu et al PLoS Biol. 2022 Sep 6;20(9):e3001772. do

Insert

pHuji-GINKO2

Expression

Insect

Promoter

hsp70 promoter

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3

Plasmid

#105802

Purpose

Concomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

ORF1: 3C-mRuby3; ORF2: TEV-mClover3

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAX_WT

Plasmid

#82888

Purpose

Gateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

MAX (MAX Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP

Plasmid

#155306

Purpose

Rfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFP

Depositor

Stanley Qi

Article

Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

Insert

Rfx Cas13d CRISPR RNA BFP

Expression

Mammalian

Promoter

hU6, EF1a

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP2K1

Plasmid

#23406

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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PB-CAG-NLS-dCas9-HA-2XNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-WPRE-PolyA-PGK-EM7-NeoR-polyA

Plasmid

#168290

Purpose

CRISPR-activator Suntag-P65-HSF1 (SPH)

Depositor

Hui Yang

Article

Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

Insert

dCas9, Suntag-P65-HSF1

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin.SF-iGluSnFR.A184V

Plasmid

#106175

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

hSynapsin

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 D614G

Plasmid

#164437

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ADRBK1

Plasmid

#23835

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADRBK1 (GRK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-RIPK2

Plasmid

#23846

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RIPK2 (RIPK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HCK

Plasmid

#23879

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HCK (HCK Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-LCK

Plasmid

#23890

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LCK (LCK Human)

Use

Gateway Cloning

Mutation

V93I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-BMPR1A

Plasmid

#23618

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR1A (BMPR1A Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pKK-TEV-BirA-FLAG

Plasmid

#105786

Purpose

Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-BirA-FLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-EPHA4

Plasmid

#23919

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

EPHA4 (EPHA4 Human)

Use

Gateway Cloning

Mutation

L335W

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK7

Plasmid

#23395

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK7 (NEK7 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-HIPK2

Plasmid

#23481

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HIPK2 (HIPK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PDK4

Plasmid

#23577

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK4 (PDK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only