Addgene

We narrowed to 17,298 results for: Eomes

Showing: 881 - 900 of 17298 results

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pCSF107mT-GATEWAY-3'-FLAG

Plasmid

#67619

Purpose

A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

C-terminal Flag

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-1

Plasmid

#92156

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-1

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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PZac2.1 gfaABC1D-Aqp4-eGFP

Plasmid

#176747

Purpose

Expresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-OPTN

Plasmid

#175760

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-OPTN

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

OPTN (OPTN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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lentiGuide-RRBP1-2

Plasmid

#92157

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3

Plasmid

#108103

Purpose

Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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Golgi-TurboID

Plasmid

#200959

Purpose

Transiently expresses golgi localized TurboID with HA tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-eGFP

Plasmid

#176862

Purpose

Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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PB-EF1-IRES-NEO mutant ITR

Plasmid

#67905

Purpose

PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

Depositor

Alex Kentsis

Article

Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

Insert

NeoR (neoR Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-3HA

Plasmid

#67616

Purpose

A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

three inframe 3' HA tags

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
V5-LOV-Turbo1-NIK3x_pTRE-G418

Plasmid

#202017

Purpose

expresses LOV-Turbo1-NIK3x in the mammalian nucleolus under dox inducible promoter, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-LOV-Turbo1-NIK3x

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

pTRE-Tight

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Expression

Mutation

Promoter

EF1a

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_Sik3

Plasmid

#119972

Purpose

Lentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Sik3 (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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Tmem214 pmRFP-N2 (1348)

Plasmid

#62047

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pLEX-FKBP-Rab11-Blasticidin

Plasmid

#120717

Purpose

Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab11a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-216

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCW57.1-ZNF746

Plasmid

#207825

Purpose

Dox-inducible expression of ZNF746

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

ZNF746 (ZNF746 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-HA_BASU

Plasmid

#153996

Purpose

Express eGFP gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

E-GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-FKBP-Rab5-Blasticidin

Plasmid

#120715

Purpose

Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab5a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Mutation

Amino acids 2-215

Promoter

CMV

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Tmem214 pEGFP-N2 (1072)

Plasmid

#62026

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

Tmem214 (TMEM214 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only