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CMV-IgK-pHluorin-TM-mRuby

Plasmid

#185546

Purpose

Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

IgK-pHluorin-TM-mRuby

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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p4A8_M59I_T94M_BC

Plasmid

#217833

Purpose

4A8 variant in barcoded, yeast surface display plasmid

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

4A8 variant Fab

Use

Tags

Expression

Yeast

Mutation

VH: M59I, VL: T94M

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV R354H SPOP 28-359

Plasmid

#197011

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Human)

Use

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

R354H

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA.3-V5-SPOP (R221C)

Plasmid

#197022

Purpose

Mammalian expression vecor for V5-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

R221C

Promoter

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA.3-V5-SPOP (R354H)

Plasmid

#197023

Purpose

Mammalian expression vecor for V5 tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

R354H

Promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroMD-mRuby2-ST

Plasmid

#171158

Purpose

Cation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroMD

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGG438

Plasmid

#165486

Purpose

Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag

Use

CRISPR

Tags

3x FLAG and SV40 NLS

Expression

Mammalian

Mutation

D1135V, S1136R, D1332K, and R1333G mutations in S…

Promoter

CMV and T7

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

Plasmid

#91806

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Residues A1529-G1534 replaced with a PreScission …

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3486 (YCp LEU2 Rpb1 F1492A)

Plasmid

#91808

Purpose

Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed phenylalanine 1492 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3487 (YCp LEU2 Rpb1 S1493A)

Plasmid

#91809

Purpose

Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed serine 1493 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3488 (YCp LEU2 Rpb1 P1494A)

Plasmid

#91810

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed proline 1494 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3508 (YCp LEU2 Rpb1 Y1473A)

Plasmid

#91811

Purpose

Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed tyrosine 1473 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3509 (YCp LEU2 Rpb1 T1471A)

Plasmid

#91812

Purpose

Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed threonine 1471 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3511 (YCp LEU2 Rpb1 P1472A)

Plasmid

#91813

Purpose

Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed proline 1472 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3567 (pET151_Spt6 1247-1451 R1282H)

Plasmid

#91816

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

changed arginine 1282 to histidine; deleted resid…

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3495 (pET151_Spt6 1247-1451 K1435A)

Plasmid

#91817

Purpose

Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

Depositor

Chris Hill

Article

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

SPT6 (SPT6 Budding Yeast)

Use

Tags

6xHis

Expression

Bacterial

Mutation

changed lysine 1435 to alanine; deleted residues …

Promoter

T7

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-HisA-HOXC6-2

Plasmid

#110365

Purpose

Mammalian expression of HisA-tagged HOXC6-2

Depositor

Carlos Moreno

Article

Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

Insert

HOXC6 (HOXC6 Human)

Use

Tags

HIS

Expression

Mammalian

Mutation

short isoform

Promoter

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6p1 AO7R

Plasmid

#137842

Purpose

GST tagged AO7 encoding aa 126-216 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pet151D-TOPO-BROX 1-401

Plasmid

#89866

Purpose

Expresses human BROX residues 1-401 in bacteria; internal ID WISP11-297

Depositor

Wesley Sundquist

Article

Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

Insert

BROX (BROX Human)

Use

Tags

His Tag, V5 tag

Expression

Bacterial

Mutation

expresses only residues 1-401

Promoter

T7

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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