We narrowed to 1,445 results for: MB

1. pNA0304

   Plasmid

   #165612

   Purpose

   Vector for expression of sgRNAs in yeast: SNR52p-NotI-sgRNA-SUP4t (NotI site in place of the spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   SNR52p-NotI-sgRNA-SUP4t (S. cerevisiae SNR52 promoter driving the sgRNA for SpCas9, with a NotI site in place of the spacer)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   NotI site replaced the CAN1 spacer in parent vect…

   Promoter

   SNR52

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGG426_UV5

   Plasmid

   #165608

   Purpose

   Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   D1135V, S1136R, D1332K and R1333G mutations in Sp…

   Promoter

   lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rat Alpha2 delta-1 mutMIDAS pMT2

   Plasmid

   #58730

   Purpose

   expression of rat alpha2 delta-1 with MIDAS site mutations in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Hoppa et al Nature. 2012 May 13;486(7401):122-5. doi

   Insert

   alpha2 delta-1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MIDAS site mutations (D259VSGS to AVAGA)

   Promoter

   SV40

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. OVA-miSFIT-2xPerfect

   Plasmid

   #124673

   Purpose

   Tuning expression of cytosolic Ovalbumin coupled to EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   Cyto-Ovalbumin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Truncated OVA for cytoplasmic expression

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-WRAP53beta-WD40domain

   Plasmid

   #64678

   Purpose

   Expresses the WD40 domain of WRAP53beta

   Depositor

   Marianne Farnebo

   Article

   Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

   Insert

   Wd40-encoding RNA antisense to P53 (WRAP53 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   containing only WD40 domain of WRAP53beta

   Promoter

   CMV

   Available since

   May 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-HisA-HOXC6-1

   Plasmid

   #110366

   Purpose

   Mammalian expression of HisA-tagged HOXC6-1

   Depositor

   Carlos Moreno

   Article

   Ramachandran et al Oncogene. 2005 Jan 6;24(1):188-98. doi:

   Insert

   HOXC6 (HOXC6 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   long isoform

   Promoter

   Available since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. OVA-miSFIT-19-G

   Plasmid

   #124676

   Purpose

   Tuning expression of cytosolic Ovalbumin coupled to EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   Cyto-Ovalbumin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Truncated OVA for cytoplasmic expression

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5 HA-GST-Presc-mAkt1 (S473T)

   Plasmid

   #48806

   Purpose

   expresses mutant Akt1

   Depositor

   David Sabatini

   Article

   Kang et al Science. 2013 Jul 26;341(6144):1236566.

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Tags

   HA-GST-Presc

   Expression

   Mammalian

   Mutation

   S473T

   Promoter

   CMV

   Available since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. OVA-miSFIT-1xPerfect

   Plasmid

   #124674

   Purpose

   Tuning expression of cytosolic Ovalbumin coupled to EGFP

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   Cyto-Ovalbumin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Truncated OVA for cytoplasmic expression

   Promoter

   minimal CMV

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. p47 PX(1-121)/p40phox(136-339)

    Plasmid

    #19018

    Depositor

    Michael Yaffe

    Article

    Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

    Insert

    p40phox/p47phox (NCF4, NCF1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert containing amino acids 1-121 of p47phox fu…

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

11. lentiGuideFE-mSG-Puro

    Plasmid

    #226522

    Purpose

    Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

    Depositor

    Neville Sanjana

    Article

    Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

    Insert

    hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    U6,EF1a

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-IgK-pHluorin-TM-mRuby

    Plasmid

    #185546

    Purpose

    Expresses surface-localized pHluorin fused to intracellular expression of mRuby under CMV promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    IgK-pHluorin-TM-mRuby
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p4A8_M59I_T94M_BC

    Plasmid

    #217833

    Purpose

    4A8 variant in barcoded, yeast surface display plasmid

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    4A8 variant Fab
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    VH: M59I, VL: T94M

    Promoter

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. His SUMO TEV R354H SPOP 28-359

    Plasmid

    #197011

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Human)
    

    Use

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    R354H

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA.3-V5-SPOP (R221C)

    Plasmid

    #197022

    Purpose

    Mammalian expression vecor for V5-tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R221C

    Promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA.3-V5-SPOP (R354H)

    Plasmid

    #197023

    Purpose

    Mammalian expression vecor for V5 tagged SPOP

    Depositor

    Tanja Mittag

    Article

    Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R354H

    Promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-ChroMD-mRuby2-ST

    Plasmid

    #171158

    Purpose

    Cation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroMD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGG438

    Plasmid

    #165486

    Purpose

    Vector for expression of the SpCas9 VRKG variant in human cells: CMV-T7-humanVRKG(SpCas9, D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFLAG

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    Mammalian codon-optimized Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G)-1xNLS(SV40)-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135V, S1136R, D1332K, and R1333G mutations in S…

    Promoter

    CMV and T7

    Available since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

    Plasmid

    #91806

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Residues A1529-G1534 replaced with a PreScission …

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CPH3486 (YCp LEU2 Rpb1 F1492A)

    Plasmid

    #91808

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed phenylalanine 1492 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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