We narrowed to 23,988 results for: Nes

1. pM-ErbB4CTF-PY1 mutant

   Plasmid

   #17798

   Depositor

   Marius Sudol

   Article

   Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

   Insert

   ErbB4 (ERBB4 Human)
   

   Use

   Tags

   GAL4-BD

   Expression

   Mammalian

   Mutation

   Carboxy-terminal fragment, amino acids 676-1292, …

   Promoter

   Available since

   May 9, 2008

   Availability

   Academic Institutions and Nonprofits only

2. p40651-EFSNS-FMR1_e1-24xms2

   Plasmid

   #222968

   Purpose

   MS2 mRNA reporter with intact FMR1 5' UTR and exon 1 sequence

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   FMR1-31CGG 5' UTR and exon1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Q54A

   Plasmid

   #217912

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP Q54A
   

   Use

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Glutamine at position 55 and 267 mutated to Alani…

   Promoter

   T5

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. T40A

   Plasmid

   #217911

   Purpose

   A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

   Depositor

   Cole DeForest

   Article

   Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

   Insert

   PXP T40A
   

   Use

   Tags

   6x His (N and C term)

   Expression

   Bacterial

   Mutation

   Threonine at position 41 and 253 mutated to Alani…

   Promoter

   T5

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM41_KPNA4_Arm

   Plasmid

   #178478

   Purpose

   Bacterial expression of human domain with His-tag and MBP tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   KPNA4_Arm (KPNA4 Synthetic, Human)
   

   Use

   Tags

   His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_ANKRA2_ANK

   Plasmid

   #178466

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   ANKRA2_ANK (ANKRA2 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM33_CRK_SH3

   Plasmid

   #178472

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CRK_SH3 (CRK Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM33_KLC1_TPR

   Plasmid

   #178477

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   KLC1_TPR (KLC1 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_CLIP_CAP_Gly

   Plasmid

   #178470

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   CLIP_CAP_Gly (CLIP1 Synthetic, Human)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM33_IRF3_SMAD/FHA

    Plasmid

    #178479

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    IRF3_IRF3 (IRF3 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGGS_cyt-AKAR5

    Plasmid

    #114138

    Purpose

    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    AKAR5 with C-terminal nuclear export signal (NES)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiMPH v2

    Plasmid

    #89308

    Purpose

    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Insert

    MS2-P65-HSF1_2A_Hygro (RELA Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N55K in MS2

    Promoter

    EF1a

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPRi(v2)-Blast

    Plasmid

    #170068

    Purpose

    Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang, Phil Sharp

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR1A FKBP12F36V-OGT

    Plasmid

    #154287

    Purpose

    Entry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiBE3-SpRY-Blast

    Plasmid

    #199303

    Purpose

    Expresses SpRY Cas9 nickase cytosine base editor FNLS-BE3 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    FNLS-BE3-SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    2A tag

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-OsTIR1(F74G)

    Plasmid

    #140730

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-NES

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G)-P2A-mAID-EGFP-NES
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139090

    Purpose

    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiRNAGuide_003

    Plasmid

    #192505

    Purpose

    For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat and smRNA, evopreQ1, capture sequences and GFP

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

    Insert

    hU6-RfxCas13d-DR1-BsmBI-DR-smRNA-SapI-CS1-evopreQ1-EFS-EGFP-2A-Puro-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM41_KPNB1_HEAT

    Plasmid

    #178494

    Purpose

    Bacterial expression of human domain with His-tag and MBP tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    KPNB1_HEAT (KPNB1 Synthetic, Human)
    

    Use

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge