We narrowed to 17,071 results for: Por

1. pAcBac1-haloTyrRS C6

   Plasmid

   #141174

   Purpose

   Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids

   Depositor

   Ryan Mehl

   Article

   Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

   Insert

   C6 HaloTyr tRNA synthetase
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   L270S, Y271L, N311G, C313T

   Promoter

   CMV

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158148

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first position of th…

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2

   Plasmid

   #191751

   Purpose

   Microtubule marker together with nuclear envelope marker driven by bidirectional promoter

   Depositor

   Jay C Dunlap

   Article

   Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

   Inserts

   NCU04288 (NCU04288 Neurospora crassa)
   bml (Bml Neurospora crassa)
   

   Tags

   Gdp1 promoter, ToxA promoter, iRFP670, and mTagBF…

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ca-LiPPI-CLAPon1.0 (APEX)

   Plasmid

   #213408

   Purpose

   Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 1)

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   MK2-E5-truncated hlov-TEVcs-APEX(201-250)-K5-HA-HA-APEX(2-200)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pONSY

   Plasmid

   #111873

   Purpose

   "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Type

   Empty backbone

   Use

   Capsaspora owczarzaki

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFGL1170R

   Plasmid

   #116896

   Purpose

   hH1:mCherry (nuclear marker)

   Depositor

   Naweed Naqvi

   Article

   Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

   Inserts

   hH1
   mCherry
   hH1 downstream region
   

   Use

   Fungal expression (in magnaporthe oryzae)

   Mutation

   no start codon, no stop codon and without start c…

   Promoter

   promoter less

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MolBC

   Plasmid

   #92210

   Purpose

   Expression of H. influenzae MolBC (ABC Transporter) in E. coli

   Depositor

   Douglas Rees

   Article

   Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

   Inserts

   MolC (HI1471 )
   MolB (HI1470 )
   

   Tags

   8x Histidine Tag and Enterokinase Cleavage Site b…

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGG-PIP68

   Plasmid

   #196806

   Purpose

   Contains SULTR4;2 promoter

   Depositor

   Marc Somssich

   Article

   Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

   Insert

   SULFATE TRANSPORTER 4.2 (SULTR4;2 Mustard Weed)
   

   Use

   Greengate (gg) entry vector

   Available Since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_rSERT_C109A_Y107C

   Plasmid

   #190174

   Purpose

   For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway

   Depositor

   Gary Rudnick

   Article

   Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.

   Insert

   SLC6A4 Serotonin Transporter (Slc6a4 Rat)
   

   Tags

   FLAG, His6, and c-myc

   Expression

   Mammalian

   Mutation

   changed Cys109 to alanine, changed tyr107 to cyst…

   Promoter

   CMV

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGB-Co-NF-kB

    Plasmid

    #180206

    Purpose

    Expression GAL4-fusion of Co-NF-kB in yeast

    Depositor

    Thomas Gilmore

    Article

    Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

    Insert

    Co-NF-kB
    

    Tags

    GAL4 (1-147) fusion

    Expression

    Yeast

    Mutation

    aa 2-1224

    Promoter

    AdhI

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8522-M01-663

    Plasmid

    #225660

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCopGFP
    

    Use

    Lentiviral

    Promoter

    SORE6-mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis-parallel1-NLSH2BCas9

    Plasmid

    #112065

    Purpose

    Expression and purification of NLSH2BCas9 for fungal gene editing

    Depositor

    Jeff Coleman

    Article

    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

    Insert

    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    

    Use

    CRISPR

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Cas9 was codon optimized for Aspergillus niger

    Promoter

    promoter in vector

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVTNT-T7-KPNA2

    Plasmid

    #26678

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 2 (KPNA2 Human)
    

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. 8522-M31-663

    Plasmid

    #225661

    Purpose

    Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    dsCherry
    

    Use

    Lentiviral

    Promoter

    SORE6-mCMVp

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMVTNT-T7-KPNA3

    Plasmid

    #26679

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 3 (KPNA3 Human)
    

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. 8138-E02

    Plasmid

    #225659

    Purpose

    Gateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)

    Depositor

    Dominic Esposito

    Article

    Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

    Insert

    SORE6
    

    Use

    Gateway entry clone

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti-CDDR

    Plasmid

    #149355

    Purpose

    CRISPR/Cas9-based dual-fluorescent DSB repair reporter

    Depositor

    Tarek Abbas

    Article

    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

    Inserts

    CDDR Reporter
    Blasticidin resistance
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a and hPGK

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPEP-TEV cNup98 (1-498)

    Plasmid

    #38037

    Depositor

    Roderick Lim

    Article

    Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

    Insert

    Nucleoporin 98 (NUP98 Human)
    

    Tags

    His-tag, 3Cysteine

    Expression

    Bacterial

    Mutation

    amino acids 1-498

    Promoter

    T7

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM9-BetaGal-8C

    Plasmid

    #172489

    Purpose

    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    M9-BetaGal-8C
    

    Tags

    6xHis and M9 nuclear localization sequence (from …

    Expression

    Bacterial

    Mutation

    BetaGal has two cysteine mutations (C77A, C1022S)…

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pONSY-mCherry

    Plasmid

    #111874

    Purpose

    This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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