Addgene

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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pRL092 pattB_cryaa:Venus_MCS

Plasmid

#213513

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Article

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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KO plasmid

Plasmid

#135970

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJYDN

Plasmid

#162450

Purpose

Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Type

Empty backbone

Use

Shuttle vector bacteria/yeast

Tags

Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pB006

Plasmid

#182320

Purpose

It's an empty replication plasmid backbone in Bacteroides

Depositor

Lei Dai

Article

Zheng et al ACS Synth Biol. 2022 Jan 21;11(1):464-47

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pmU1-CasRx-non-targeting (pKW83)

Plasmid

#229051

Purpose

Cloning backbone for CasRx crRNA (Mouse U1 promoter)

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRpHLuorin2-N1

Plasmid

#171716

Purpose

Ratiometric pHLuorin2 backbone (C terminal fusion)

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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mOrange2-N1

Plasmid

#54568

Purpose

Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA6 1397N

Plasmid

#221939

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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FusX alphaDdCBE DddA11 1397N

Plasmid

#221940

Purpose

Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397N

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Type

Empty backbone

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mutation

TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pkk223_Null

Plasmid

#213111

Purpose

pkk223 backbone plasmid

Depositor

Martin Horvath

Article

Utzman et al PLoS One. 2024 May 8;19(5):e0284642. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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mOrange2-C1

Plasmid

#54650

Purpose

Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

Depositor

Michael Davidson, Roger Tsien

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pHC79

Plasmid

#167551

Purpose

Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Type

Empty backbone

Use

Cosmid

Tags

Expression

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII

Plasmid

#162750

Purpose

MSCV backbone

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJUMP45-2A(sfGFP)

Plasmid

#126989

Purpose

Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCOLA-Spec-EV

Plasmid

#212095

Purpose

(Empty Backbone) pCOLA-Spec Empty Vector

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-empty-pgk-hph

Plasmid

#48753

Purpose

PiggyBac backbone, CAG promoter, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Type

Empty backbone

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only
pUdOm2.3

Plasmid

#210280

Purpose

Empty backbone with ampicillin resistance for intermediate expression in mammalian cells

Depositor

Corrie daCosta

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pASR103

Plasmid

#122637

Purpose

BglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniae (pPEPZ Backbone)

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pHA1

Plasmid

#85709

Purpose

This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Martin Messerle, Ulrich Koszinowski

Article

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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