We narrowed to 2,059 results for: gem

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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Publication

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Publication

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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promoter J23110 (upstream of RBS, mRFP1)

Plasmid

#117852

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

medium-high promoter, RBS, mRFP1

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pZeo-Cas9-gate

Plasmid

#113744

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.

Depositor

Magdalena Bezanilla

Publication

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR and Gateway Cloning

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJC5-4-insulator-BREreporter-insulator

Plasmid

#171499

Purpose

injections to make Bmp reporter mouse transgenic line

Depositor

James Briscoe

Publication

Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

Insert

Insulator BRE-MLP:H2B-Turqoise

Expression

Mammalian

Promoter

BRE-MLP

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only
pFBD-Akt13C(S473D)

Plasmid

#86577

Purpose

Expresses cleavable human full-length Akt1(S473D) phosphomimetic

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Tags

10xHis

Expression

Insect

Mutation

3C cleavage site inserted at pos. 130; S473D phos…

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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pJRTCLCrVB1.3

Plasmid

#22844

Depositor

Niki Robertson

Publication

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

B DNA

Use

Cotton vigs vector

Expression

Bacterial

Mutation

1.3 unit B componet length inserted into pBlueScr…

Available since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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pISH-En2

Plasmid

#84310

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

En2 (En2 Mouse)

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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BBa_J72110-BBa_J72094

Plasmid

#40778

Depositor

Christopher Anderson

Publication

Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

Insert

AraC-pBad + coi

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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XP3 Xwnt-4

Plasmid

#16988

Depositor

Randall Moon

Insert

Xwnt-4 (wnt4 Frog)

Expression

Bacterial

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pSB1A3_GFP_dT33

Plasmid

#89668

Purpose

expression of doubleTAL staple protein dT33 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT33

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Msx1

Plasmid

#84313

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx1 (Msx1 Mouse)

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PK187

Plasmid

#44623

Depositor

Robert Oshima

Publication

Oshima et al Differentiation. 1986;33(1):61-8.

Insert

K18 (KRT18 Human)

Use

Cloning

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pMB1-A

Plasmid

#190114

Purpose

Level 1 MoClo plasmid for A. baumannii

Depositor

Veronica Godoy-Carter

Publication

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNP3614 [pNP3613 - ISCro4 Recombinase (WT)]

Plasmid

#247265

Purpose

Express ISCro4 recombinase

Depositor

Patrick Hsu

Publication

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP3613 - ISCro4 Recombinase (WT)

Expression

Mammalian

Available since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRS305-LEU2-pINO4-SV40NLS-PfV-GS-yEmCherry

Plasmid

#203656

Purpose

Yeast integrative vector for the expression of the yEmCherry-tagged nuclear localizing 40nm nucGEMs under the INO4 promoter.

Depositor

Liam Holt

Publication

Shu T et et al bioRxiv 2022

Insert

PfV

Tags

yEmCherry

Expression

Yeast

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMOR256-17-ISH

Plasmid

#15528

Depositor

Peter Mombaerts

Publication

Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

Insert

MOR256-17 (Olfr15 Mouse)

Use

For riboprobe, in situ hybridization

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pTU2-a-RFP

Plasmid

#72958

Purpose

Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB

Depositor

Paul Freemont

Publication

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 2 - a site

Use

Synthetic Biology

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only
pMOR22-2-ISH

Plasmid

#15839

Depositor

Peter Mombaerts

Publication

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR22-2 (Olfr69 Mouse)

Use

Cloning vector

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pMOR31-2-ISH

Plasmid

#15844

Depositor

Peter Mombaerts

Publication

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

MOR31-2 (Olfr690 Mouse)

Use

Cloning vector

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only