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Plasmid#159469PurposeEncodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-Kss1
Plasmid#52682PurposeExpression of Kss1 in bacteriaDepositorInsertKSS1 (KSS1 Budding Yeast)
UseTagsHis6 and T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-A'-l4-G'
Plasmid#209124PurposeBacterial expression of CC-GEMS ligand SUMO-A'-l4-G'DepositorInsertSUMO-A'-l4-G'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL + STS
Plasmid#139790Purposeexpression of biosynthetic enzymes 4CL and STSDepositorInserts4CL
STS
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_STRAW
Plasmid#204240PurposeBacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_STRAW (mdh Streptomyces avermitilis)
UseTags6X HisExpressionBacterialMutationPromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG
Plasmid#176545PurposeExpresses IgG affinity protein FB with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
UseTagsHis tagExpressionBacterialMutationchange glutamic acid 25 to amber stop codonPromoterT5Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET31b-T7-Spinach2-Ct
Plasmid#79158PurposeExpresses the Ct biosensor under a T7 promoterDepositorInsertT7-tRNA-Spinach2-Ct
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-sfGFP
Plasmid#180757PurposepET30 vector missing lacO and N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET23a RanGAP
Plasmid#13387DepositorInsertRanGAP (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.0
Plasmid#81050Purposebacterial expression of murine TDGDepositorInsertTDG (Tdg Mouse)
UseTags6HISExpressionBacterialMutationPromoterT7Available sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-R4-uAb
Plasmid#101801PurposeBacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAbDepositorInsertR4-uAb
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET23b-proMMP-7
Plasmid#29524DepositorInsertproMMP-7 (MMP7 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET15b/BOA
Plasmid#82041PurposeFor E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)DepositorInsertBurkholderia oklahomensis agglutinin
UseTags6x HisExpressionBacterialMutationPromoterAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET14b PHAS-I
Plasmid#15679DepositorInsertPHAS-I (Eif4ebp1 Rat)
UseTags6xHisExpressionBacterialMutationNucleotides 1-354 of rat PHAS-I plus 450bp of 3…PromoterAvailable sinceSept. 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF4n_mFAP2b
Plasmid#159464PurposeEncodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF4n_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF108_3xFLAG_YPet
Plasmid#106441PurposepUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsisDepositorInsertZF108_3xFLAG_YPet
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
YPet-H2B-6
Plasmid#56632PurposeLocalization: Histone H2B, Excitation: 513, Emission: 527DepositorInsertH2B (H2BC11 Human)
UseTagsYPetExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceDec. 19, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET30b-7D-SrtA
Plasmid#105600Purposeexpresses 7D sortase A variant in E.coliDepositorInsertS.aureus Sortase A, 7D mutant
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1p3_mFAP2b
Plasmid#159470PurposeEncodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p3_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-mFAP10
Plasmid#159463PurposeEncodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertmFAP10
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only