We narrowed to 9,995 results for: pET

Showing: 881 - 900 of 9995 results

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pET-(pos36)GFP-9xGGS-Cre-6xHis

Plasmid

#62938

Purpose

Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(ps36)GFP-9xGGS-Cre

Tags

6xHis tag and (pos36)GFP

Expression

Bacterial

Promoter

T7

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pET-His6MBP-A3A-Gly6His6

Plasmid

#187822

Purpose

Expression human APOBEC3A in E.coli.

Depositor

Hao Wu

Publication

Fabyanic et al Nat Biotechnol. 2023 Aug 28. doi: 10.103

Insert

APOBEC3A (APOBEC3A Human)

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

none

Promoter

T7 promoter (on the backbone)

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Publication

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PET-HiFi SpCas9-NLS-6xHis

Plasmid

#207376

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R691A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-BMTU UDG-6xHis

Plasmid

#172197

Purpose

expresses His-tagged uracil-DNA-glycosylase enzyme from marine bacterium BMTU 3346 for the purpose of cross contamination prevention in nucleic acid amplification reactions

Depositor

Andrea Pauli

Insert

BMTU UDG

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-His6-Sumo-CfaC-MBP

Plasmid

#126018

Purpose

Bacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)

Depositor

Thomas Muir

Insert

His6-Sumo-CfaC-MBP

Tags

6xHis-SUMO and HA-MBP

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-15b-hAR-663-919

Plasmid

#89083

Purpose

bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

Depositor

Elizabeth Wilson

Publication

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR ligand binding domain (AR Human)

Tags

His

Expression

Bacterial

Mutation

aa 663-919

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HOXC9 (murine) HIS-tag pET

Plasmid

#8559

Depositor

Corey Largman

Insert

HOXC9 (Hoxc9 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Western

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Publication

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Tags

His-tagged

Expression

Bacterial

Promoter

T7 promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-HsSENP3

Plasmid

#71467

Purpose

Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Publication

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp9 C287A-His

Plasmid

#11830

Depositor

Guy Salvesen

Publication

Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

Insert

Caspase 9 catalytic mutant (CASP9 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C287A

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28a-mH6-Cas12c1

Plasmid

#120872

Purpose

Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET_scFv_QLuc

Plasmid

#183261

Purpose

Express scFv_QLuc in bacteria cells

Depositor

Huiwang Ai

Publication

Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

Insert

scFv_QLuc

Tags

Histag

Expression

Bacterial

Promoter

T7

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Publication

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET:CCCT:GFP

Plasmid

#60738

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET:ACAT:GFP

Plasmid

#60740

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET:GCAT:GFP

Plasmid

#60736

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

Depositor

Matthew Bennett

Publication

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pET-Duet1_6xHis-TEV-eGFP-P62

Plasmid

#190929

Purpose

Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

Depositor

Sascha Martens

Publication

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

P62 (SQSTM1 Human)

Tags

EGFP

Expression

Bacterial

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-ALFA-His

Plasmid

#171569

Purpose

Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Publication

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-ALFA-His (UBE2J1 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-hTBP

Plasmid

#171073

Purpose

Expresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBP

Depositor

Joan Conaway

Publication

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TATA-box-binding protein (TBP Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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