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Showing: 8981 - 9000 of 9094 results

  1. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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  2. P530_SIX6-p2A-h2b-EGFP

    Plasmid
    #239101
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Article
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    SIX6 (SIX6 Human)
    Use
    Donor plasmid
    Tags
    p2A-h2b-EGFP
    Expression
    Mammalian
    Promoter
    Endogenous SIX6 promoter
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239101-plasmid-map-sequence-id-474411

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  3. G protein alpha-o-YFP

    Plasmid
    #55776
    Purpose
    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Mammalian
    Mutation
    A BglII site was removed from the 5' untrans…
    Promoter
    CMV
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55776-plasmid-map-sequence-id-112541

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  5. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156430
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    Cterm_Nterm_switched
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156430-plasmid-map-sequence-id-310203

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  7. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid
    #171802
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-mgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171802-plasmid-map-sequence-id-337973

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  8. MADR pDonor TagBFP2-3XFlag (cyto) WPRE

    Plasmid
    #129421
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of a TagBFP2-3XFlag cytoplasmic reporter protein
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-3Flag
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    3X Flag epitope tag
    Promoter
    none (3X polyA to mitigate episomal expression)
    Available Since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129421-plasmid-map-sequence-id-257066

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  10. Spike Display_Part 2 DO

    Plasmid
    #172722
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 2 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172722-plasmid-map-sequence-id-344857

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  11. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid
    #171801
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-hgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171801-plasmid-map-sequence-id-337972

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  13. pLVX-Myc-HECT(C2007)-Puro

    Plasmid
    #216784
    Purpose
    Myc-HECT(TRIP12); the HECT domain (AA 1651-2040) of TRIP12 with an N-terminal Myc-tag & a puromycin resistance cassette; AA C2007 changed to A
    Depositor
    Robert Sobol
    Article
    Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do
    Insert
    Myc-HECT(TRIP12) (TRIP12 Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    HECT domain (amino acids 1651-2040); aa C2007 cha…
    Available Since
    Jan. 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    216784-plasmid-map-sequence-id-498159

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  14. pLVX-Myc-TRIP12-SB-Puro

    Plasmid
    #216769
    Purpose
    Myc-TRIP12-SB; the substrate binding (SB) domain (AA 1-1650) of TRIP12 with an N-terminal Myc-tag & a puromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do
    Insert
    Myc-TRIP12-SB (TRIP12 Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Substrate binding domain of TRIP12 (amino acids 1…
    Available Since
    Jan. 26, 2026
    Availability
    Academic Institutions and Nonprofits only
    216769-plasmid-map-sequence-id-498168

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Showing: 8981 - 9000 of 9094 results