162,325 results

1. pSF1389

   Plasmid

   #104962

   Purpose

   Bacterial expression plasmid of bdSENP1 protease

   Depositor

   Dirk Görlich

   Article

   Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

   Insert

   SENP1
   

   Use

   Tags

   His14 tag and Tev protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   lacUV5

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSM019

   Plasmid

   #128291

   Purpose

   asymmetric plasmid partitioning E. coli - regulatory plasmid

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   Para - lp-sfyfp-parB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HDM-tat1b

   Plasmid

   #204154

   Purpose

   Lentivirus helper plasmid coding for Tat

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   tat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

   Plasmid

   #85756

   Purpose

   All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Inserts

   Csy4-T2A-SpRFN-P2A-GFP
   gRNA
   

   Use

   CRISPR

   Tags

   Csy4, Csy4 recognition sequence, FokI fusion via …

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CMV and U6

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Anti-TrpC1 [1F1R]

   Plasmid

   #177437

   Purpose

   Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFCiKm7

   Plasmid

   #167521

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinRAM-KmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-32a(+)-TGFB1m

   Plasmid

   #120286

   Purpose

   Produce human recombinant mTGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β1
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promotor

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPJ23116-MS2-rep

   Plasmid

   #184842

   Purpose

   Expression of the rep protein of phage MS2

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Replicase gene MS2 phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23116

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE 80L Kan T7WT

    Plasmid

    #102790

    Purpose

    Expresses T7 RNA polymerase

    Depositor

    Andrew Ellington

    Article

    Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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