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pFLIPglu-3.2mDelta13

Plasmid

#12992

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A S112A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A, S112A in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3-Xl-VSP1-GFP

Plasmid

#51883

Purpose

expression of Xl-VSP1 -GFP fusion in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 (tpte2 Frog)

Use

Xenopus oocyte expression

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab5c

Plasmid

#109651

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5c

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5c (rab5c Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11ba

Plasmid

#109596

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11ba (rab11ba Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pML104-kanR1136

Plasmid

#162040

Purpose

Expresses Cas9 and contains kanR1136 guide which targets the kanR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

kanR1136 guide

Use

CRISPR

Expression

Yeast

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pFGL1079

Plasmid

#116897

Purpose

CenpC:eGFP (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Cenp C (partial)
eGFP
CenpC downstream region

Use

Fungal expression (in magnaporthe oryzae)

Mutation

truncated, no stop codon and without start codon

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHB4

Plasmid

#53462

Purpose

This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

Depositor

Elizabeth Conibear

Article

Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

Insert

yeGFP-Snc1-Suc2

Expression

Yeast

Promoter

ADH

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-EHD2a

Plasmid

#109516

Purpose

Muscle specific zebrafish expression of mKate2 fused to EHD2a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD2a (ehd2a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-170nDelta13

Plasmid

#12989

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16F Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

WT binding protein (no F16A)

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pUASp-Egl-Trbo

Plasmid

#179993

Purpose

Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

Egl-Trbo

Tags

TurboID

Expression

Insect

Promoter

UAS-Gal4

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab34a

Plasmid

#109625

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab34a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab34a (rab34a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF3∆165N (WT)

Plasmid

#175419

Purpose

Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE662

Plasmid

#153260

Purpose

M6 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE664

Plasmid

#153262

Purpose

M7 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE659

Plasmid

#153257

Purpose

M4 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE661

Plasmid

#153259

Purpose

M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-actn2-mKate2

Plasmid

#109751

Purpose

Muscle specific zebrafish expression of mKate2 fused to alpha actinin 2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

alpha actinin 2

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3

Plasmid

#79613

Purpose

Expresses dCas9-SH3 in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

SH3 domain

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_1

Plasmid

#169885

Purpose

Disrupt ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgACO1_2

Plasmid

#169892

Purpose

Disrput ACO1

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting ACO1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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