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pMIG-hNFATc1/bC

Plasmid

#74049

Purpose

retroviral expression plasmid for human NFATc1/bC

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

human NFATc1, isoform beta-C (NFATC1 Human)

Use

Retroviral

Tags

no tag

Expression

Mammalian

Promoter

pMSCV-LTRs

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
pACT1:Cas9-GFP, U6:sgTK

Plasmid

#122852

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum TK gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgTK

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317 YAP1

Plasmid

#193683

Purpose

Constitutive lentiviral expression of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-Luciferase-hvTK-bla

Plasmid

#179450

Purpose

Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

Luciferase

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF1

Plasmid

#141188

Purpose

Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF1 (GIGYF1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3F-COMP5AP-AviTag-9xHis

Plasmid

#157541

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3F (SEMA3F Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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STT3A pEGFP-N2 (1051)

Plasmid

#62025

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
TetO-FUW-Ezh2

Plasmid

#118587

Purpose

Expresses Ezh2 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Foxo1

Plasmid

#118588

Purpose

Expresses Foxo1 from the TRE3G promoter

Depositor

Stanley Qi

Article

Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

Insert

Foxo1 (Foxo1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317 CALM2

Plasmid

#193684

Purpose

Constitutive lentiviral expression of CALM2

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

CALM2 (CALM2 Human)

Use

Lentiviral

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMIA3

Plasmid

#109399

Purpose

Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB

Depositor

Roger Foo

Article

Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d

Type

Empty backbone

Use

CRISPR

Tags

mRuby2 and mtp53dn

Expression

Mammalian

Promoter

EF1a

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mScarlet-I-CD4-bla

Plasmid

#179449

Purpose

Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mClover3-CD4-bla

Plasmid

#179448

Purpose

Donor vector to knock in mClover3 N-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mClover3

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR FL CPAP RNAi resistant

Plasmid

#46390

Depositor

Pierre Gonczy

Article

Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

Insert

CPAP RNAi resistant mutant (CENPJ Human)

Use

Entry vector

Mutation

silent mutation that renders it resistant to siRN…

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only
pPHR-EYFP-hHP1β

Plasmid

#103829

Purpose

Soluble BLInCR effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-hHP1β (CRY2, CBX1 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pACT1:Cas9-GFP, U6:sgUPRT

Plasmid

#122853

Purpose

Expresses Cas9 fused with GFP and a sgRNA targeting the Cryptosporidium parvum UPRT gene

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

Cas9-GFP
U6-sgUPRT

Use

CRISPR; Cas9-gfp plasmid for genome editing of cr…

Tags

eGFP

Promoter

Cryptosporidium parvum U6 and Cryptosporidium par…

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Ikka-HA (K44M)

Plasmid

#23297

Depositor

Warner Greene

Article

Geleziunas et al Mol Cell Biol. 1998 Sep . 18(9):5157-65.

Insert

IKK alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Mutation

K44M

Available Since

April 12, 2010

Availability

Academic Institutions and Nonprofits only
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

Plasmid

#122085

Purpose

Express M.EcoGII fused to CENPC with destabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)

Use

Retroviral

Tags

V5 tag on Nter of CENPC

Expression

Bacterial and Mammalian

Mutation

L341F, R930L (see comments below)

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-E55A-ATPIF1

Plasmid

#85404

Purpose

Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase

Depositor

David Sabatini

Article

Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

Insert

ATPIF1 (ATP5IF1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

E55A point mutation abrogating the ability of ATP…

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only