We narrowed to 12,197 results for: 112

1. pETSUMO2_Bacteroidetes-bGSDM

   Plasmid

   #212124

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bacteroidetes bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSUMO2_Runella-bGSDM

   Plasmid

   #212123

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Runella bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETSUMO2_Vitiosangium-bGSDM

   Plasmid

   #212127

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Vitiosangium bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETSUMO2_Gillisia-bGSDM

   Plasmid

   #212128

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Gillisia bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. IKK2-EYFP

   Plasmid

   #111196

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

   Insert

   IKKbeta (IKBKB Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA_DiCre_60.F2

   Plasmid

   #60208

   Purpose

   Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

   Depositor

   Jean-Paul Herman

   Article

   Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

   Insert

   Cre recombinase (CD207 Bacteriophage P1, Human, Synthetic)
   

   Tags

   FRAP and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   amino acids 2021-2112 of FRAP with a T2098L mutat…

   Promoter

   CMV

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS1516

   Plasmid

   #205979

   Purpose

   FLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas7 from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SaBE3-Gam

   Plasmid

   #100810

   Purpose

   Expresses SaBE3-Gam in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

   Insert

   SaBE3-Gam
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-mGold-Actin-C-18

   Plasmid

   #158009

   Purpose

   Visualization of actin in mammalian cells using mGold

   Depositor

   Francois St-Pierre

   Article

   Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

   Insert

   Actin (ACTB Human)
   

   Tags

   mGold

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTU425

    Plasmid

    #178984

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT H585A C627A
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CoV2-N-D3L

    Plasmid

    #177945

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (D3L) (N )
    

    Expression

    Mammalian

    Mutation

    D3L

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

    Plasmid

    #115137

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy, Keith Joung

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2.1 anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CoV2-N-A119S

    Plasmid

    #177948

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (A119S) (N )
    

    Expression

    Mammalian

    Mutation

    A119S

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-alpha4 wt (Hygro)

    Plasmid

    #80017

    Purpose

    Mammalian expression of human integrin alpha4, Hygro selection

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    integrin alpha4 (ITGA4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pET-51b(+)-Caprola_14

    Plasmid

    #194675

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_14
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-51b(+)-Caprola_15

    Plasmid

    #194676

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_15
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTorPE-GEM-GECO1

    Plasmid

    #32463

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Lab constructed

    Tags

    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9-EF-Gsk3b(new)

    Plasmid

    #122341

    Purpose

    Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Gsk3b
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXs_FLAG-SFXN5

    Plasmid

    #110639

    Purpose

    Retroviral expression of Flag-SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC010 LshCas13a locus with nontargeting spacer

    Plasmid

    #91901

    Purpose

    LshCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with non-targeting spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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